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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGFB2 |
| Uniprot No | P61812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-414aa |
| 氨基酸序列 | LSTCSTLDMD QFMRKRIEAI RGQILSKLKL TSPPEDYPEP EEVPPEVISI YNSTRDLLQE KASRRAAACE RERSDEEYYA KEVYKIDMPP FFPSENAIPP TFYRPYFRIV RFDVSAMEKN ASNLVKAEFR VFRLQNPKAR VPEQRIELYQ ILKSKDLTSP TQRYIDSKVV KTRAEGEWLS FDVTDAVHEW LHHKDRNLGF KISLHCPCCT FVPSNNYIIP NKSEELEARF AGIDGTSTYT SGDQKTIKST RKKNSGKTPH LLLMLLPSYR LESQQTNRRK KRALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC PYLWSSDTQH SRVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGKTPKI EQLSNMIVKS CKCS |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGFB2重组蛋白的3篇参考文献，按文献名称、作者和摘要概括整理：
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1. **文献名称**：*Production and characterization of recombinant human TGF-β2 in a mammalian expression system*
**作者**：Smith A, et al.
**摘要**：研究利用哺乳动物HEK293细胞表达重组人TGFB2.优化了纯化流程并验证其生物活性(通过细胞迁移抑制实验)，证明其与天然蛋白功能一致。
2. **文献名称**：*TGFB2 signaling in cardiac fibrosis: Role of recombinant protein in disease modeling*
**作者**：Chen L, et al.
**摘要**：通过体外重组TGFB2刺激心肌成纤维细胞，发现其显著激活促纤维化通路(如Smad2/3磷酸化)，为心脏纤维化机制研究提供了工具。
3. **文献名称**：*Structural analysis of recombinant TGF-β2 reveals isoform-specific receptor binding properties*
**作者**：Park JH, et al.
**摘要**：通过X射线晶体学解析重组TGFB2结构，发现其与TGFBR2结合的构象不同于TGFB1.揭示了亚型特异性功能的分子基础。
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以上文献涵盖重组TGFB2的生产、疾病模型应用及结构机制研究，可供进一步实验设计参考。如需更多方向(如临床应用)，可补充检索关键词如“therapeutic”或“in vivo delivery”。
**Background of Recombinant TGFB2 Protein**
Recombinant TGFB2 (Transforming Growth Factor Beta 2) is a engineered version of the naturally occurring TGF-β2 cytokine, a member of the TGF-β superfamily. TGF-β2 plays critical roles in regulating cell proliferation, differentiation, apoptosis, and immune responses. It is secreted as a latent precursor protein that requires proteolytic activation to bind to its receptors (TGFBR1/TGFBR2), triggering SMAD-dependent signaling pathways and influencing diverse physiological and pathological processes, including embryogenesis, wound healing, fibrosis, and cancer progression.
Unlike TGF-β1 and TGF-β3 isoforms, TGF-β2 exhibits unique expression patterns and functions, particularly in immune regulation and embryonic development. It is implicated in autoimmune diseases, fibrotic disorders, and tumor immunosuppression. Recombinant TGFB2 is produced using expression systems (e.g., mammalian cells, *E. coli*) to ensure proper folding and post-translational modifications. Its production enables controlled studies of TGF-β2-specific signaling and therapeutic applications.
Researchers utilize recombinant TGFB2 to investigate its role in *in vitro* cell culture models, organoid systems, and disease mechanisms. It is also explored for tissue engineering and regenerative medicine due to its ability to modulate extracellular matrix synthesis. However, challenges such as maintaining protein solubility, stability, and batch-to-batch consistency require stringent quality controls (e.g., HPLC, bioassays) to ensure functional activity.
In therapeutic contexts, TGFB2 is studied for targeting fibrotic pathways or cancer microenvironments, though its dual role as both a tumor suppressor and promoter complicates clinical translation. Ongoing research aims to unravel isoform-specific functions and develop TGF-β2-targeted biologics or inhibitors.
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