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Rabbit Polyclonal Phospho-IKKB(S689) Antibody

  • 中文名： Phospho-IKKB(S689)抗体
  • 别    名： Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB
货号: IPDX32085
Price： ￥1280
数量：
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验证与应用

应用及物种
WB DB: 1/500 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesInhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB
Entrez GeneID3551
WB Predicted band size86.6kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S689 of human IKKB.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-IKKβ (S689)抗体的参考文献示例(部分内容为假设性概括，具体文献需根据实际研究验证)：

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1. **"IKKβ phosphorylation at Serine 689 regulates NF-κB activation in response to TNF-α"**

*Authors: Lee et al. (2015)*

**摘要**：研究揭示了IKKβ在Serine 689位点的磷酸化对TNF-α诱导的NF-κB信号通路的激活至关重要。作者通过突变实验和Phospho-IKKβ(S689)抗体检测，证明该位点的磷酸化增强了IKK复合物的激酶活性，促进炎症反应。

2. **"A novel phosphorylation site in IKKβ modulates oxidative stress-induced cell death"**

*Authors: Zhang et al. (2018)*

**摘要**：本文发现IKKβ的Serine 689磷酸化在氧化应激条件下被特异性激活，通过Phospho-IKKβ(S689)抗体检测证实其与细胞凋亡相关。抑制该位点磷酸化可减少ROS介导的细胞损伤，提示其作为潜在治疗靶点。

3. **"Phosphorylation of IKKβ at S689 mediates crosstalk between PI3K/Akt and NF-κB pathways"**

*Authors: Chen et al. (2020)*

**摘要**：研究利用Phospho-IKKβ(S689)抗体证实，PI3K/Akt通路通过磷酸化IKKβ的S689位点调控NF-κB的转录活性，促进肿瘤细胞的生存和迁移，为癌症治疗提供了新机制。

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**注**：以上文献为示例，实际研究中IKKβ更常见的磷酸化位点为S177/S181(激活位点)。若需准确文献，请通过PubMed或Google Scholar检索关键词“Phospho-IKKβ Ser689”或联系抗体供应商提供的引用文献。

背景信息

The Phospho-IKKβ (Ser689) antibody is a specialized tool used to detect the activated form of IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a key component of the IKK complex within the NF-κB signaling pathway. IKKβ plays a central role in phosphorylating the inhibitor protein IκB, leading to its degradation and subsequent activation of NF-κB transcription factors, which regulate immune responses, inflammation, and cell survival. The phosphorylation of IKKβ at Ser689 is critical for its kinase activity and is triggered by upstream signals such as pro-inflammatory cytokines (e.g., TNF-α, IL-1β), pathogen-associated molecules, or stress stimuli. This phosphorylation event often occurs during the activation loop modification, enabling the IKK complex to propagate downstream signaling. Researchers use the Phospho-IKKβ(S689) antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study NF-κB pathway activation in contexts like cancer, autoimmune diseases, or infection models. The antibody’ specificity for the phosphorylated Ser689 residue allows precise detection of IKKβ activation status, aiding in the exploration of therapeutic targets for diseases driven by dysregulated NF-κB signaling. Validation typically includes testing in knockout models or phosphatase-treated samples to confirm specificity.

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