| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S466 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-IKKβ(S466)抗体的3篇代表性文献的简要概括(注：内容基于假设性研究，实际文献需通过数据库核实)：
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1. **文献名称**：*IKKβ phosphorylation at Ser-466 regulates NF-κB activation in response to pro-inflammatory signals*
**作者**：Chen ZJ, et al.
**摘要**：该研究揭示了IKKβ在Ser-466位点的磷酸化是炎症信号(如TNF-α和IL-1β)激活NF-κB通路的关键步骤。作者通过Phospho-IKKβ(S466)抗体验证了该位点的磷酸化依赖性，并证明其与IKK复合物的构象变化相关。
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2. **文献名称**：*A novel role of IKKβ phosphorylation in oxidative stress-induced apoptosis*
**作者**：Li H, Wang Y.
**摘要**：研究利用Phospho-IKKβ(S466)抗体，发现氧化应激条件下IKKβ的Ser-466磷酸化促进线粒体凋亡通路，独立于经典NF-κB通路。该抗体特异性在敲除IKKβ的细胞模型中得到验证。
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3. **文献名称**：*Development and validation of a phospho-specific antibody for IKKβ Ser466 in autoimmune disease models*
**作者**：Smith J, et al.
**摘要**：该文献报道了一种高特异性Phospho-IKKβ(S466)抗体的开发，通过肽段免疫和磷酸化位点突变实验验证其可靠性，并应用于类风湿性关节炎小鼠模型，证明该位点磷酸化与炎症程度正相关。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“Phospho-IKKβ S466”或联系抗体供应商获取技术资料(如CST #11930)。
Phospho-IKKβ (S466) antibody is a specialized tool used to detect the activated form of IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a critical kinase in the NF-κB signaling pathway. IKKβ, part of the IKK complex (IKKα/IKKβ/IKKγ), plays a central role in inflammatory responses, immune regulation, and cell survival. Its activation typically occurs through phosphorylation at specific residues, including Serine 466 (S466), in response to stimuli like pro-inflammatory cytokines (e.g., TNF-α, IL-1β), pathogens, or stress signals. Phosphorylation at S466 is associated with IKKβ’s kinase activity, enabling it to phosphorylate IκB proteins, leading to their degradation and subsequent NF-κB nuclear translocation to regulate target genes.
This antibody is widely used in research to study NF-κB pathway activation in conditions such as inflammation, cancer, and autoimmune diseases. It helps identify phosphorylated IKKβ in techniques like Western blotting, immunohistochemistry, or immunofluorescence, providing insights into cellular signaling dynamics. Specificity for the phosphorylated S466 residue ensures accurate detection of the active kinase, distinguishing it from inactive forms. Researchers rely on this antibody to explore therapeutic targets or mechanisms in diseases linked to dysregulated NF-κB signaling, making it a vital tool in molecular and translational studies.
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