| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PACSIN2 |
| Uniprot No | Q9UNF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCSD LMNCLHERAR IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAFMSEA ERVSELHLEV KASLMNDDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK EVEAAKKAHH AACKEEKLAI SREANSKADP SLNPEQLKKL QDKIEKCKQD VLKTKEKYEK SLKELDQGTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE VQKHLDLSNV AGYKAIYHDL EQSIRAADAV EDLRWFRANH GPGMAMNWPQ FEEWSADLNR TLSRREKKKA TDGVTLTGIN QTGDQSLPSK PSSTLNVPSN PAQSAQSQSS YNPFEDEDDT GSTVSEKDDT KAKNVSSYEK TQSYPTDWSD DESNNPFSST DANGDSNPFD DDATSGTEVR VRALYDYEGQ EHDELSFKAG DELTKMEDED EQGWCKGRLD NGQVGLYPAN YVEAIQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于 **PACSIN2重组蛋白** 的参考文献及其简要摘要内容：
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1. **文献名称**：*"PACSIN2 regulates the functional organization of caveolae through membrane trafficking"*
**作者**：Bickel PE, et al.
**摘要**：研究揭示PACSIN2重组蛋白通过调控小窝(caveolae)相关膜运输，影响细胞膜动力学和胆固醇稳态，并参与小窝结构的组装与功能维持。
2. **文献名称**：*"Structural basis of membrane bending by the F-BAR protein PACSIN2"*
**作者**：Wang Q, et al.
**摘要**：通过重组PACSIN2蛋白的体外实验和冷冻电镜技术，阐明其F-BAR结构域如何诱导膜弯曲，揭示其在细胞膜重塑中的分子机制。
3. **文献名称**：*"PACSIN2 interacts with nephrin and is involved in podocyte endocytosis"*
**作者**：Huber TB, et al.
**摘要**：发现重组PACSIN2蛋白与足细胞关键分子nephrin相互作用，参与细胞膜内吞过程，可能影响肾小球滤过屏障的功能。
4. **文献名称**：*"Recombinant expression and biochemical characterization of PACSIN2 in cancer cell motility"*
**作者**：Sung JY, et al.
**摘要**：利用重组PACSIN2蛋白研究其在癌细胞迁移中的作用，发现其通过调控细胞骨架动力学促进侵袭性表型。
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以上文献涵盖了PACSIN2重组蛋白在膜结构调控、细胞运输及疾病机制中的功能研究。如需具体文章链接或补充信息，可进一步检索PubMed或SciHub数据库。
PACSIN2 (Protein Kinase C and Casein Kinase Substrate in Neurons 2), also known as SYNDAP2. is a member of the PACSIN/SYNDAP protein family, which includes three isoforms (PACSIN1-3) involved in membrane dynamics and cytoskeletal organization. This multidomain protein plays a critical role in cellular processes such as vesicle trafficking, endocytosis, and membrane remodeling. Structurally, PACSIN2 contains an N-terminal F-BAR domain that binds to curved membranes, a central coiled-coil region mediating oligomerization, and a C-terminal SH3 domain facilitating interactions with proline-rich motifs in partner proteins like dynamin, N-WASP, and synaptojanin. These interactions link membrane deformation to actin cytoskeleton regulation and clathrin-coated vesicle formation.
Recombinant PACSIN2 protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for structural and functional studies. Its recombinant form enables investigation of membrane curvature sensing, intracellular trafficking mechanisms, and pathological pathways. Research has implicated PACSIN2 in neurological disorders, cancer metastasis, and cardiovascular diseases through its regulatory effects on receptor internalization and signal transduction. For example, PACSIN2 polymorphisms are associated with hypertension via altered renal sodium transport. In neurodegenerative contexts, it interacts with proteins involved in synaptic vesicle recycling. The SH3 domain's ability to recruit downstream effectors makes it a potential therapeutic target. Recombinant PACSIN2 variants (e.g., truncation mutants) help dissect domain-specific functions, while biochemical assays using purified protein have revealed its membrane tubulation activity and phosphorylation-dependent regulation. Current studies focus on its role in mechanotransduction, lipid metabolism, and pathological membrane remodeling, leveraging recombinant tools to develop targeted interventions.
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