| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BLNK |
| Uniprot No | Q8WV28 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BLNK重组蛋白的参考文献示例(注：文献标题和作者为示例性虚构，仅供参考实际文献需根据数据库检索确认)：
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1. **文献名称**：*"Recombinant BLNK protein rescues BCR signaling in BLNK-deficient B cells"*
**作者**：Fu, C., Watanabe, D., Kurosaki, T.
**摘要**：研究通过在大肠杆菌中表达重组BLNK蛋白，证实其在BLNK缺陷的B细胞中恢复B细胞受体(BCR)介导的钙离子内流及MAPK激活，揭示了BLNK在信号转导中的支架作用。
2. **文献名称**：*"Crystal structure of the SH2 domain complex of BLNK and its interaction partners"*
**作者**：Xu, S., Hsu, E., Chan, A.C.
**摘要**：利用重组BLNK蛋白的SH2结构域进行晶体学研究，解析其与下游分子(如PLCγ2)的结合模式，阐明了BLNK在信号复合体组装中的分子机制。
3. **文献名称**：*"Recombinant BLNK suppresses B cell hyperactivation in murine lupus models"*
**作者**：Jiang, L., Peng, Q., Tsokos, G.C.
**摘要**：在小鼠系统性红斑狼疮模型中，外源性重组BLNK蛋白通过调控B细胞异常活化减轻了自身抗体产生，提示其潜在治疗应用价值。
4. **文献名称**：*"Optimized expression and purification of functional human BLNK in insect cells"*
**作者**：Smith, R.P., Collins, K.L., Clark, M.R.
**摘要**：报道一种基于杆状病毒-昆虫细胞系统的高效BLNK重组表达策略，获得高纯度蛋白用于体外磷酸化及相互作用分析。
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**备注**：以上文献为模拟内容，实际引用时需通过PubMed、Web of Science等数据库检索真实文献(建议关键词：BLNK/SLP-65. recombinant protein, B cell signaling)。知名研究者如Tomohiro Kurosaki、Anthony C. Chan等可能有相关论文。
BLNK (B-cell linker protein), also known as SLP-65 or BASH, is a critical adaptor protein in B-cell receptor (BCR) signaling. Discovered in the late 1990s, it plays a central role in bridging BCR activation to downstream intracellular pathways. Structurally, BLNK contains multiple modular domains, including an N-terminal leucine zipper, a proline-rich region, and a C-terminal SH2 domain, which facilitate interactions with kinases (e.g., Syk, BTK) and effector molecules (e.g., PLCγ, Vav). Upon BCR engagement, BLNK is phosphorylated, forming a signaling scaffold that coordinates calcium mobilization, MAPK activation, and transcriptional regulation, essential for B-cell development, proliferation, and antibody production.
Recombinant BLNK proteins are engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, functional forms for research. These proteins retain post-translational modification sites (e.g., tyrosine phosphorylation) and interaction domains, enabling studies on BCR signaling mechanisms. BLNK-deficient models exhibit impaired B-cell maturation and immune responses, linking its dysfunction to immunodeficiencies and B-cell malignancies. In drug discovery, recombinant BLNK aids in screening therapeutics targeting B-cell disorders. Recent advances include structural studies resolving its dynamic assembly in signalosomes and CRISPR-edited BLNK variants to dissect pathway specificity. Its role in autoimmune diseases and lymphoma continues to drive translational interest.
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