| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMX2 |
| Uniprot No | Q9Y320 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 125-296aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTCKPPLYMGPEYIKYFNDKTIDEELERDK RVTWIVEFFANWSNDCQSFAPIYADLSLKYNCTGLNFGKVDVGRYTDVST RYKVSTSPLTKQLPTLILFQGGKEAMRRPQIDKKGRAVSWTFSEENVIRE FNLNELYQRAKKLSKAGDNIPEEQPVASTPTTVSDGENKKDK |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMX2重组蛋白的3篇参考文献概览(文献为虚拟示例，仅供参考)：
1. **文献名称**：*TMX2重组蛋白在氧化应激中的功能研究*
**作者**：Zhang Y, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化了TMX2重组蛋白，发现其通过硫氧还蛋白活性调控内质网氧化应激，并证实其与PDI互作参与错误折叠蛋白的修复。
2. **文献名称**：*TMX2结构解析及其跨膜结构域的作用机制*
**作者**：Lee S, et al.
**摘要**：利用昆虫细胞系统表达TMX2重组蛋白，通过冷冻电镜解析其三维结构，揭示了其跨膜域如何通过构象变化传递氧化还原信号至内质网膜。
3. **文献名称**：*TMX2重组蛋白在神经退行性疾病模型中的保护作用*
**作者**：Garcia R, et al.
**摘要**：在阿尔茨海默病细胞模型中，外源性添加纯化的TMX2重组蛋白显著减少tau蛋白聚集，提示其可能通过调节内质网应激延缓神经元损伤。
(注：以上为模拟内容，实际文献需通过PubMed等学术平台检索关键词如“TMX2 recombinant protein”或“TMX2 protein function”获取。)
TMX2 (Thioredoxin-related transmembrane protein 2) is a member of the thioredoxin (Trx) protein family, characterized by a conserved C-terminal Trx-like domain with redox-active motifs (typically CXXC). Unlike classical cytosolic thioredoxins, TMX2 is anchored to membranes via an N-terminal transmembrane domain, primarily localizing to the endoplasmic reticulum (ER) and mitochondrial-associated membranes. It plays roles in redox regulation, protein folding, and quality control within these compartments.
As a protein disulfide isomerase (PDI)-like chaperone, TMX2 participates in disulfide bond formation/rearrangement during nascent protein folding. It interacts with ER-resident oxidoreductases and may modulate ER stress responses, calcium homeostasis, and autophagy. TMX2 also exhibits context-dependent roles in apoptosis and cell survival, potentially influencing cancer progression and neurodegenerative diseases.
Recombinant TMX2 protein is engineered for in vitro studies to dissect its biochemical properties, substrate specificity, and interactions. Produced via bacterial or mammalian expression systems, it often includes affinity tags (e.g., His-tag) for purification. Research applications include enzymatic assays (e.g., insulin disulfide reduction), structural analysis (X-ray crystallography, NMR), and functional studies in cellular models. Its redox activity and ER-mitochondria crosstalk make it a target for investigating metabolic disorders, oxidative stress-related pathologies, and therapeutic development.
Current studies focus on TMX2's dual role in redox signaling and its interplay with ER stress sensors like IRE1α. Challenges remain in elucidating tissue-specific functions and post-translational modifications regulating its activity.
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