| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BLMH |
| Uniprot No | Q13867 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSSGLNSEKVAALIQKLNSDPQFVLAQNV GTTHDLLDICLKRATVQRAQHVFQHAVPQEGKPITNQKSS GRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYLFFWDKVERCYFFLSAFVD TAQRKEPEDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVIPKKCFPESYT TEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BLMH(Bleomycin Hydrolase)重组蛋白的参考文献及简要摘要：
---
1. **文献名称**: *Crystal Structure of Human Bleomycin Hydrolase: A Self-Compartmentalizing Protease*
**作者**: Joshua Z. Montelione, et al.
**摘要**: 本研究解析了人源BLMH重组蛋白的晶体结构，揭示了其独特的六聚体自隔离结构特征。该结构表明BLMH通过中央通道限制底物结合，解释了其对博莱霉素等细胞毒性药物的水解机制，为靶向药物设计提供了结构基础。
---
2. **文献名称**: *Recombinant Expression and Functional Characterization of Bleomycin Hydrolase in Drug Resistance*
**作者**: Maria T. Müller, et al.
**摘要**: 通过在大肠杆菌中重组表达BLMH蛋白，研究证实其能够有效降解博莱霉素并降低药物诱导的DNA损伤。实验进一步发现BLMH过表达与肿瘤细胞对博莱霉素的耐药性相关，提示其在化疗敏感性调控中的潜在作用。
---
3. **文献名称**: *Role of BLMH in Neurodegenerative Disorders: Insights from Recombinant Protein Studies*
**作者**: Hiroshi Kikuchi, et al.
**摘要**: 利用重组BLMH蛋白进行体外酶活分析，发现其可水解β-淀粉样蛋白前体片段。研究推测BLMH可能通过调节淀粉样蛋白代谢影响阿尔茨海默病的病理进程，为神经退行性疾病机制研究提供了新视角。
---
*注：以上文献信息为示例，实际引用时需核实具体论文及作者。建议通过PubMed或Web of Science以“Bleomycin Hydrolase recombinant”为关键词检索最新研究。*
BLMH (Bleomycin Hydrolase) is a conserved cytosolic cysteine protease that plays diverse roles in cellular processes, including drug metabolism, protein homeostasis, and neurodegenerative disease pathways. Initially identified for its ability to detoxify the anticancer glycopeptide bleomycin by hydrolyzing its β-aminoalanine moiety, BLMH has since been linked to broader biological functions. It is ubiquitously expressed in human tissues, with high activity in the liver, kidneys, and brain. Structurally, BLMH belongs to the papain superfamily, featuring a catalytic triad (Cys, His, Asn) and a unique C-terminal extension critical for substrate recognition.
Recombinant BLMH protein is widely used to study its enzymatic mechanisms and pathophysiological relevance. In cancer research, it serves as a model to investigate chemoresistance mechanisms, as elevated BLMH levels correlate with reduced bleomycin efficacy. In neuroscience, BLMH's role in degrading neurotoxic peptides like β-amyloid (implicated in Alzheimer's disease) has spurred interest in its regulatory pathways. Recombinant expression systems (e.g., E. coli, mammalian cells) enable production of active BLMH for biochemical assays, inhibitor screening, and structural studies using X-ray crystallography. Recent studies also explore its involvement in glutathione metabolism and oxidative stress responses. Despite progress, questions remain about its endogenous substrates and tissue-specific regulation, driving ongoing demand for recombinant BLMH tools in both basic and therapeutic research.
×
