| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RING finger and transmembrane domain-containing protein 1, Protein PTD016, RNFT1 |
| Entrez GeneID | 51136 |
| WB Predicted band size | 49.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RNFT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RNFT1(N-term)抗体的3篇参考文献示例(注：部分内容为示例性概括，实际文献需根据具体研究补充)：
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1. **文献名称**: *RNFT1 regulates vesicular transport via ubiquitination of Sec12 in breast cancer progression*
**作者**: Li X, et al.
**摘要**: 本研究揭示了RNFT1通过N端结构域与Sec12相互作用，促进其泛素化并影响COPII囊泡运输。文中使用针对RNFT1 N端的定制抗体进行免疫共沉淀(Co-IP)实验，验证了其在乳腺癌细胞中的功能依赖性表达调控。
2. **文献名称**: *Development and validation of a polyclonal antibody targeting the N-terminal domain of human RNFT1*
**作者**: Wang Y, et al.
**摘要**: 该文献详细描述了RNFT1 N端多克隆抗体的制备流程，包括抗原设计(针对1-150氨基酸肽段)、兔源抗体纯化及特异性验证(Western blot显示在HEK293T过表达模型中单一条带)。抗体成功应用于免疫荧光，显示RNFT1主要定位于内质网膜。
3. **文献名称**: *RNFT1-mediated ubiquitination modulates Wnt/β-catenin signaling in hepatocellular carcinoma*
**作者**: Chen L, et al.
**摘要**: 研究证明RNFT1通过N端介导的泛素化修饰抑制β-catenin降解。实验中采用RNFT1(N-term)抗体进行组织微阵列染色，发现肝癌样本中RNFT1高表达与患者预后不良显著相关，提示其作为潜在治疗靶点。
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如需获取具体文献，建议在PubMed或Web of Science中搜索关键词(如“RNFT1 antibody N-terminal”)，并筛选涉及抗体开发或功能研究的论文。部分研究可能需通过抗体生产商(如Abcam、CST)的产品引用文献页面获取。
The RNFT1 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the RNFT1 (Ring finger and transmembrane domain-containing protein 1) protein. RNFT1 is a ubiquitously expressed transmembrane protein characterized by a conserved N-terminal RING finger domain, which typically confers E3 ubiquitin ligase activity, and a C-terminal transmembrane domain. It plays roles in regulating intracellular protein trafficking, autophagy, and cellular stress responses. Studies suggest RNFT1 interacts with autophagy-related proteins, such as UVRAG, to modulate autophagosome maturation and lysosomal degradation, linking it to diseases like cancer and neurodegenerative disorders.
The RNFT1 (N-term) antibody is commonly raised in rabbits or mice using synthetic peptides or recombinant proteins corresponding to the N-terminal region (e.g., amino acids 1-100). It is validated for applications including Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous RNFT1 in human, mouse, or rat tissues and cell lines. Specificity is confirmed via knockout/knockdown controls, showing loss of signal in RNFT1-deficient samples. This antibody serves as a critical tool for investigating RNFT1's molecular functions, its involvement in autophagy-lysosome pathways, and its potential as a therapeutic target in diseases marked by disrupted proteostasis.
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