| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGOLN2 |
| Uniprot No | O43493 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-381aa |
| 氨基酸序列 | ATESVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSS AEAQTPEDTPNKSGAEAKTQKDSSNKSGAEAKTQKGSTSKSGSEAQTTKD STSKSHPELQTPKDSTGKSGAEAQTPEDSPNRSGAEAKTQKDSPSKSGSE AQTTKDVPNKSGADGQTPKDGSSKSGAEDQTPKDVPNKSGAEKQTPKDGS NKSGAEEQGPIDGPSKSGAEEQTSKDSPNKVVPEQPSRKDHSKPISNPSD NKELPKADTNQLADKGKLSPHAFKTESGEETDLISPPQEEVKSSEPTEDV EPKEAEDDDTGPEEGSPPKEEKEKMSGSASSENREGTLSDSTGSEKDDLY PNGSGNGSAEVDHHHHHH |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGOLN2重组蛋白的3条假设性参考文献(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Recombinant TGOLN2 expression in Escherichia coli and its role in Golgi-mediated protein sorting"*
**作者**: Zhang, Y. et al.
**摘要**: 报道了在大肠杆菌中高效表达可溶性TGOLN2重组蛋白的方法，并验证其通过调控Rab蛋白活性参与高尔基体囊泡运输的功能。
2. **文献名称**: *"Structural and functional characterization of human TGOLN2 as a key regulator of endosomal trafficking"*
**作者**: Müller, C. & Schmidt, R.
**摘要**: 通过X射线晶体学解析TGOLN2重组蛋白结构，发现其C端结构域与Arf1蛋白互作，调控内吞体-高尔基体逆向运输通路。
3. **文献名称**: *"TGOLN2 recombinant protein as a novel biomarker in colorectal cancer diagnosis"*
**作者**: Li, X. et al.
**摘要**: 利用重组TGOLN2蛋白开发特异性抗体，发现其在结直肠癌组织中异常高表达，提示其作为潜在诊断标志物的可能性。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science或Google Scholar等平台检索具体文献。建议使用关键词“TGOLN2 recombinant protein”、“TGN2/TGN46 protein”或“Trans-Golgi network protein 2”进行精准查询。
TGOLN2 (Trans-Golgi Network Protein 2), also known as Golgin-45. is a peripheral membrane protein localized to the trans-Golgi network (TGN), a critical cellular hub for sorting and trafficking secretory and lysosomal proteins. It belongs to the golgin family, characterized by coiled-coil domains that mediate interactions with other proteins and membranes. TGOLN2 plays a role in maintaining Golgi structure, vesicle tethering, and cargo transport. Studies suggest its involvement in glycosylation processes, autophagy, and cellular response to stress. Dysregulation of TGOLN2 has been linked to diseases, including cancers and neurodegenerative disorders, highlighting its functional significance in cellular homeostasis.
Recombinant TGOLN2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian or insect cell) systems, the recombinant form retains specific functional domains, enabling researchers to analyze protein-protein interactions, post-translational modifications, and structural features. Its production often incorporates affinity tags (e.g., His-tag) for purification. Applications include elucidating TGOLN2’s role in secretory pathways, autophagy regulation, and pathological contexts like tumor metastasis, where aberrant Golgi function is observed. Recombinant TGOLN2 also serves as an antigen for antibody development, aiding diagnostic and therapeutic research. Overall, it is a vital tool for advancing understanding of Golgi biology and disease mechanisms.
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