| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BLK |
| Uniprot No | P51451 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-505aa |
| 氨基酸序列 | MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPP PPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSL VTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLI RESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQA LVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSG QFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLY AVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAY IERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIK WTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLER GYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQ YELQPLE |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BLK重组蛋白的参考文献示例(注：部分信息为模拟，仅供参考)：
1. **文献名称**：*Structural insights into the catalytic mechanism of BLK tyrosine kinase through recombinant protein crystallography*
**作者**：Cowan-Jacob SW, et al.
**摘要**：本研究通过重组表达并纯化人源BLK激酶结构域，利用X射线晶体学解析其三维结构，揭示了ATP结合口袋的构象特征，为设计选择性激酶抑制剂提供了结构基础。
2. **文献名称**：*Autoimmune-associated BLK variants impair B cell receptor signaling via altered recombinant protein stability*
**作者**：Hashimoto S, et al.
**摘要**：文章通过体外表达携带红斑狼疮相关突变的BLK重组蛋白，发现其稳定性降低导致B细胞受体信号传导异常，揭示了BLK在自身免疫疾病中的分子机制。
3. **文献名称**：*High-throughput screening of BLK inhibitors using a recombinant kinase assay system*
**作者**：Namchuk M, et al.
**摘要**：研究构建了基于重组BLK蛋白的体外激酶活性检测体系，筛选出小分子化合物库中的潜在抑制剂，为治疗B细胞恶性肿瘤提供候选药物。
4. **文献名称**：*BLK interacts with STAT3 via its SH2 domain: Evidence from recombinant protein pull-down assays*
**作者**：Lopes J, et al.
**摘要**：通过重组表达BLK及STAT3蛋白并进行体外结合实验，证实BLK的SH2结构域直接介导与STAT3的相互作用，调控JAK-STAT信号通路在B细胞分化中的功能。
**注**：以上文献信息为示例性模拟，实际研究需以具体数据库(如PubMed)检索结果为准。建议通过关键词“BLK recombinant protein”、“BLK kinase”或“BLK structure/function”查询最新文献。
BLK (B lymphoid tyrosine kinase) is a member of the Src family of non-receptor tyrosine kinases, primarily expressed in B cells and involved in intracellular signaling pathways. It plays a critical role in B cell receptor (BCR) signaling, influencing cell proliferation, differentiation, and immune response regulation. BLK activation occurs through phosphorylation events triggered by antigen binding to BCRs, initiating downstream cascades involving proteins like Syk, PI3K, and MAP kinases. Dysregulation of BLK has been linked to autoimmune diseases (e.g., systemic lupus erythematosus, rheumatoid arthritis) and hematological malignancies, highlighting its dual role in normal immunity and pathology.
Recombinant BLK proteins are engineered using expression systems like *E. coli*, insect cells, or mammalian cell lines to produce functional or truncated forms for research. These proteins retain kinase activity and phosphorylation sites, enabling studies on signaling mechanisms, protein interactions, and inhibitor screening. Purification techniques often involve affinity tags (e.g., GST, His-tag) followed by chromatography. Researchers use recombinant BLK to dissect its structural domains—SH3. SH2. and catalytic kinase—and explore mutations affecting enzymatic activity or disease associations.
In drug discovery, recombinant BLK serves as a target for kinase inhibitors, particularly in autoimmune and cancer therapies. Its role in modulating immune tolerance and aberrant signaling makes it a focus for therapeutic intervention. Recent studies also explore BLK's expression in non-B cells (e.g., dendritic cells) and its broader regulatory functions. Overall, BLK recombinant proteins are vital tools for unraveling B cell biology and developing precision treatments.
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