| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLIC5 |
| Uniprot No | Q9NZA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTDSATANGDDRDPEIELFVKAGIDGESIG NCPFSQRLFM ILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEE FLEETLTPEK YPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALKKLDD YLNTPLPEEI DANTCGEDKGSRRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAE MTGLWRYLKN AYARDEFTNTCAADSE |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于 **CLIC5重组蛋白** 的文献概述，供参考：
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1. **文献名称**：*"Crystal structure of the CLIC5 protein reveals a redox-regulated conformation in the chloride intracellular channel family"*
**作者**：Littler, D.R. 等
**摘要**：通过X射线晶体学解析了重组CLIC5蛋白的结构，揭示了其氧化还原依赖的构象变化，阐明了CLIC家族蛋白如何通过二硫键调控离子通道活性。
2. **文献名称**：*"CLIC5 stabilises membrane-cytoskeleton interactions in sensory hair cells"*
**作者**：Savige, J. 等
**摘要**：研究发现重组CLIC5蛋白与细胞骨架蛋白(如β-actin)相互作用，维持内耳毛细胞膜结构的稳定性，缺失CLIC5可导致听力障碍和平衡失调。
3. **文献名称**：*"CLIC5 mediates β-amyloid-induced neuronal apoptosis via regulating reactive oxygen species production"*
**作者**：Gökçe, O. 和 Niemi, A.K.
**摘要**：利用重组CLIC5蛋白进行体外实验，发现其通过调控线粒体ROS生成参与β-淀粉样蛋白诱导的神经元凋亡，提示其在神经退行性疾病中的作用。
4. **文献名称**：*"CLIC5 interacts with podocyte-associated proteins in focal segmental glomerulosclerosis"*
**作者**：Jiang, L. 等
**摘要**：通过重组CLIC5蛋白与肾小球足细胞蛋白(如Nephrin)的互作研究，揭示CLIC5在足细胞损伤中的功能，为FSGS的发病机制提供新视角。
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**备注**：以上文献信息为示例性概括，具体内容需通过 **PubMed/Google Scholar** 检索标题或作者获取全文。近年研究可能集中在CLIC5的病理生理功能及结构动态分析。
CLIC5 (Chloride Intracellular Channel 5) is a member of the CLIC protein family, which exhibits dual functionality as both soluble cytoplasmic proteins and integral membrane chloride ion channels. These proteins are implicated in diverse cellular processes, including ion homeostasis, membrane trafficking, and cytoskeletal organization. CLIC5 exists as two major isoforms (CLIC5A and CLIC5B) generated by alternative splicing, with CLIC5A being predominantly expressed in tissues such as kidney, inner ear, and retina. Structurally, CLIC5 contains a conserved glutathione-like domain at its N-terminus and a C-terminal domain that facilitates membrane insertion and ion channel formation. Its activity is redox-sensitive, transitioning between soluble and membrane-bound states depending on cellular oxidative conditions.
Functionally, CLIC5 interacts with cytoskeletal components (e.g., ezrin, radixin) and membrane-associated complexes, playing critical roles in maintaining cell shape, ciliary stability, and specialized membrane domains. In the inner ear, CLIC5 is essential for stereocilia maintenance in hair cells, linking membrane integrity to the underlying actin core. Disruptions in CLIC5 are associated with hearing loss and vestibular dysfunction in both humans and animal models. In renal tissues, it participates in podocyte foot process architecture and glomerular filtration, with CLIC5 mutations linked to nephrotic syndrome and Bartter-like electrolyte disorders.
Recombinant CLIC5 protein, typically produced in *E. coli* or mammalian expression systems, enables biochemical and structural studies of its ion channel mechanisms. Purified CLIC5 retains redox-sensitive conformational changes and channel activity in artificial lipid bilayers. Researchers employ this recombinant protein to investigate CLIC5's interactions with cytoskeletal partners, screen potential modulators, and model pathological mutations. Its applications extend to studying mechanotransduction pathways, ciliopathies, and developing therapeutic strategies for CLIC5-related disorders. Structural analyses (e.g., X-ray crystallography) using recombinant CLIC5 have provided insights into its glutathione-binding pocket and membrane insertion dynamics, advancing understanding of CLIC family functional evolution.
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