| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAT1 |
| Uniprot No | Q6PIZ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-186aa |
| 氨基酸序列 | MNISHYVEKQRQDKMYSYSSDHTRVDEYYIEDTPIYGNLDDMISEPMDEN CYEQMKARPEKSVNKMQEATPSAQATNETQMCYASLDHSVKGKRRKPRKQ NTHFSDKDGDEQLHAIDASVSKTTLVDSFSPESQAVEENIHDDPIRLFGL IRAKREPIN |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRAT1重组蛋白的3篇参考文献示例(注：因文献检索限制，以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *TRAT1: A novel adapter protein involved in TCR signaling*
**作者**: Wang Y, et al.
**摘要**: 本研究通过重组表达TRAT1蛋白，揭示了其在T细胞受体(TCR)信号传导中的作用。实验表明，重组TRAT1可与CD3ζ链直接相互作用，并通过调控下游激酶活性影响T细胞活化。该研究为TCR信号通路的分子机制提供了新见解。
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2. **文献名称**: *Expression and functional analysis of recombinant TRAT1 in immune synapse formation*
**作者**: Müller S, et al.
**摘要**: 作者利用哺乳动物表达系统成功制备了重组TRAT1蛋白，并证明其在免疫突触形成中起关键作用。通过体外结合实验，发现TRAT1通过稳定TCR-CD3复合物增强信号转导效率，为免疫治疗靶点开发提供了依据。
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3. **文献名称**: *Structural insights into TRAT1-mediated T cell activation using recombinant protein crystallography*
**作者**: Li H, et al.
**摘要**: 本研究通过重组TRAT1蛋白的结晶和结构解析，揭示了其跨膜适配功能的结构基础。结果显示，TRAT1的胞内域具有独特的螺旋构象，可能作为支架蛋白募集下游信号分子，解释了其在T细胞激活中的关键作用。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“TRAT1 recombinant protein”或“TRAT1 signaling”检索近期研究。
TRAT1 (T-cell receptor-associated transmembrane adaptor 1) is a transmembrane protein primarily involved in T-cell receptor (TCR) signaling and immune regulation. It belongs to the transmembrane adaptor protein (TRAP) family, which lacks enzymatic activity but serves as a scaffold to recruit signaling molecules. TRAT1 interacts with components of the TCR-CD3 complex, particularly CD3ζ and CD3ε chains, facilitating the assembly and stabilization of the TCR signaling module. This interaction is critical for propagating downstream signaling events, including calcium flux, kinase activation, and cytokine production, which are essential for T-cell activation, proliferation, and immune response coordination.
Recombinant TRAT1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional forms of the protein for experimental studies. Its recombinant form often includes tags (e.g., His, GST) for ease of purification and detection. Researchers utilize TRAT1 recombinant protein to dissect its structural and functional roles in TCR signaling, particularly in mapping binding partners, characterizing post-translational modifications, and studying immune synapse formation. Additionally, it serves as a tool to investigate immune dysregulation mechanisms in autoimmune diseases (e.g., lupus, rheumatoid arthritis) or T-cell malignancies, where TCR signaling pathways may be altered.
Recent studies suggest TRAT1 may influence immune checkpoint regulation, linking it to cancer immunotherapy research. Its recombinant variants are also explored for therapeutic targeting, such as modulating T-cell activity in adoptive cell therapies. Despite progress, the full scope of TRAT1's interactions and regulatory mechanisms remains under investigation, highlighting the importance of recombinant protein tools in advancing this field.
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