| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLIC3 |
| Uniprot No | O95833 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MAETKLQLFVKASEDGESVGHCPSCQRLFMVLLLKGVPFTLTTVDTRRSP DVLKDFAPGSQLPILLYDSDAKTDTLQIEDFLEETLGPPDFPSLAPRYRE SNTAGNDVFHKFSAFIKNPVPAQDEALYQQLLRALARLDSYLRAPLEHEL AGEPQLRESRRRFLDGDRLTLADCSLLPKLHIVDTVCAHFRQAPIPAELR GVRRYLDSAMQEKEFKYTCPHSAEILAAYRPAVHPRLEHHHHHH |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLIC3重组蛋白的模拟参考文献示例(注：文献为虚构概括，实际研究请通过学术数据库查询)：
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1. **"CLIC3 Recombinant Protein Promotes Tumor Cell Invasion via pH Regulation"**
- **作者**: Smith A, et al.
- **摘要**: 研究通过表达并纯化CLIC3重组蛋白，发现其通过调节溶酶体pH值增强肿瘤细胞的侵袭能力，机制涉及与V-ATPase的相互作用。
2. **"Structural Characterization of Recombinant CLIC3 and Its Ion Channel Activity"**
- **作者**: Lee J, et al.
- **摘要**: 利用X射线晶体学解析CLIC3重组蛋白的结构，揭示其依赖氧化还原状态的构象变化，并在脂质体中验证其氯离子通道功能。
3. **"CLIC3 in Endosomal Trafficking: Insights from Recombinant Protein Knockdown Models"**
- **作者**: Chen R, et al.
- **摘要**: 通过重组CLIC3体外实验及基因敲除细胞模型，证明CLIC3通过调控Rab GTPases影响内体膜运输，影响细胞迁移和分泌通路。
4. **"Recombinant CLIC3 as a Therapeutic Target in Pancreatic Cancer"**
- **作者**: Wang Y, et al.
- **摘要**: 研究发现CLIC3重组蛋白在胰腺癌中高表达，抑制其活性可减少小鼠模型中肿瘤转移，提示其作为治疗靶点的潜力。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词“CLIC3 recombinant protein”获取真实文献。如需具体文章协助，请提供更多信息!
CLIC3 (Chloride Intracellular Channel 3) is a member of the CLIC family of proteins, which exhibit dual functionality as both soluble cytoplasmic proteins and integral membrane chloride ion channels. Structurally, CLIC3 contains a conserved glutathione S-transferase (GST)-like domain with redox-sensitive properties, enabling its transition between monomeric and dimeric states. This conformational flexibility allows CLIC3 to participate in cellular processes such as ion homeostasis, membrane trafficking, and organelle acidification. Unlike other CLIC members, CLIC3 shows distinct subcellular localization, predominantly associating with endosomal-lysosomal compartments and secretory vesicles, where it regulates chloride flux critical for vesicular maturation and cargo transport.
Recombinant CLIC3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships and biochemical properties. The recombinant form often includes affinity tags (e.g., His-tag) for purification and tracking. Research highlights its role in cancer progression, particularly in tumor invasion and metastasis, where CLIC3-mediated chloride transport facilitates extracellular matrix remodeling and cell migration. It also interacts with signaling pathways involving mTOR and TGF-β, linking ion channel activity to cell proliferation and apoptosis.
Additionally, CLIC3 has implications in autophagy and neurodegenerative diseases, as its ion channel activity influences lysosomal function. Despite progress, its exact gating mechanism and physiological triggers remain debated, partly due to its redox-dependent structural dynamics. Recombinant CLIC3 serves as a tool to explore these mechanisms and validate its potential as a therapeutic target or biomarker in pathologies like cancer and lysosomal storage disorders. Current challenges include resolving its membrane-insertion process and reconciling discrepancies between *in vitro* studies and *in vivo* biological roles.
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