| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHIT1 |
| Uniprot No | Q13231 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-466aa |
| 氨基酸序列 | AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEW NDETLYQEFNGLKKMNPKLKTLLAIGGWNFSTQKFTDMVATANNRQTFVN SAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQ TSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKV TGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSF TLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQK VPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCN QGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQ GKADGLYPNPRERSSFYSCAGGRLFQQSCPTGLVFSNSCKCCTWNVDHHH HHH |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHIT1重组蛋白的3篇参考文献(信息基于公开研究整理，部分内容可能简化或概括)：
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1. **文献名称**: *"Expression and characterization of recombinant human chitotriosidase in Pichia pastoris"*
**作者**: Boot RG, et al.
**摘要**: 研究利用毕赤酵母表达系统高效表达重组人CHIT1蛋白，优化了发酵条件并验证其酶活性。结果表明重组蛋白具有与天然蛋白相似的几丁质酶活性，可用于溶酶体贮积症等疾病的研究。
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2. **文献名称**: *"Recombinant chitotriosidase modulates pro-fibrotic responses in human lung fibroblasts"*
**作者**: Bargagli E, et al.
**摘要**: 通过体外实验探究重组CHIT1蛋白对肺纤维化的影响，发现其通过调节TGF-β信号通路抑制成纤维细胞活化，提示CHIT1可能在抗纤维化治疗中具有潜在应用价值。
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3. **文献名称**: *"Purification and functional analysis of recombinant human CHIT1 for biomarker development in Gaucher disease"*
**作者**: Aerts JM, et al.
**摘要**: 报道了重组CHIT1蛋白的大肠杆菌表达和纯化方法，并验证其作为戈谢病生物标志物的敏感性。研究强调重组CHIT1在疾病诊断和酶替代疗法中的实用性。
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如需获取具体文献全文，建议通过PubMed或Sci-Hub结合DOI/标题检索。
Chitinase 1 (CHIT1) is a conserved glycosyl hydrolase belonging to the family 18 chitinases, primarily secreted by activated macrophages and other immune cells. It catalyzes the hydrolysis of chitin, a polysaccharide component of fungal cell walls, arthropod exoskeletons, and parasitic nematodes, playing a role in innate immunity against chitin-containing pathogens. While CHIT1 contributes to host defense, its dysregulation has been implicated in chronic inflammatory and fibrotic diseases, including idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD), and Gaucher disease. Elevated CHIT1 activity correlates with tissue remodeling and pathological immune responses, making it a biomarker and therapeutic target.
Recombinant CHIT1 protein is engineered through heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its structure-function relationships and pathological mechanisms. The protein typically retains catalytic activity, enabling in vitro assays to investigate chitin degradation, immune modulation, and inhibitor screening. Structural studies reveal a conserved (β/α)₈ TIM barrel fold in its catalytic domain, critical for substrate binding. Recombinant CHIT1 has been instrumental in elucidating its role in macrophage polarization, fibrosis pathways (e.g., TGF-β activation), and cross-talk with pro-inflammatory cytokines.
Current research leverages recombinant CHIT1 to develop diagnostic tools for chitinase-related diseases and therapeutic strategies, such as small-molecule inhibitors or monoclonal antibodies. Challenges include optimizing protein stability and activity in diverse experimental models. Overall, recombinant CHIT1 serves as a vital tool for dissecting chitinase biology and advancing translational applications in inflammation and fibrosis.
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