| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YKT6 |
| Uniprot No | O15498 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MKLYSLSVLYKGEAKVVLLKAAYDVSSFSFFQRSSVQEFMTFTSQLIVER SSKGTRASVKEQDYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDE FSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETK IILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIMLD LQSR |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YKT6重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*"YKT6 is a core component of membrane fusion machinery in autophagy and vesicle trafficking"*
**作者**：Takashi Yoshihara 等
**摘要**：研究揭示了YKT6作为SNARE蛋白家族成员，在自噬体和溶酶体膜融合中的关键作用。通过重组蛋白实验，验证了YKT6与其他SNARE蛋白(如STX17和SNAP29)的相互作用，并证明其重组形式在体外可驱动膜融合。
2. **文献名称**：*"Structural insights into YKT6-mediated vesicle fusion in neuronal exocytosis"*
**作者**：Maria S. Rodriguez 等
**摘要**：该研究解析了重组YKT6蛋白的晶体结构，发现其N端长in结构域对膜锚定至关重要。通过体外囊泡融合实验，证实重组YKT6与Syntaxin-1A/SNAP-25形成复合体，调控神经递质释放。
3. **文献名称**：*"Recombinant YKT6 expression enhances exosome secretion in cancer cells"*
**作者**：Li Chen 等
**摘要**：通过在大肠杆菌中表达重组YKT6蛋白，研究发现其过表达可促进肿瘤细胞外泌体的分泌。实验表明，YKT6通过调控多泡体与质膜的融合，影响肿瘤微环境中的细胞通讯。
(注：以上文献为示例性概括，实际文献需通过学术数据库检索。)
**Background of YKT6 Recombinant Protein**
YKT6 is a member of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) family, which plays a critical role in intracellular membrane fusion events. These processes are essential for vesicle trafficking, organelle maintenance, and secretion pathways. YKT6 is classified as a v-SNARE (vesicle-associated SNARE) that interacts with specific t-SNAREs (target membrane-associated SNAREs) to mediate the fusion of transport vesicles with target membranes. It is highly conserved across eukaryotes and is involved in diverse cellular functions, including autophagy, Golgi-endoplasmic reticulum transport, and synaptic vesicle release in neurons.
The recombinant YKT6 protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity chromatography, yielding high-purity protein for research applications. Recombinant YKT6 retains the functional SNARE domains required for binding partner interactions, enabling studies on its structural and mechanistic roles in membrane dynamics.
Research on YKT6 has gained attention due to its implications in neurodegenerative diseases, cancer, and metabolic disorders. Dysregulation of YKT6-mediated trafficking is linked to disrupted autophagy in Alzheimer’s and Parkinson’s diseases, as well as abnormal secretion pathways in tumors. Additionally, studies using recombinant YKT6 have elucidated its role in viral infection mechanisms, where pathogens hijack SNARE complexes for cell entry or replication.
Overall, recombinant YKT6 serves as a vital tool for dissecting membrane fusion biology and developing therapeutic strategies targeting trafficking-related pathologies. Its study bridges fundamental cell biology with translational medicine, highlighting its importance in both physiological and disease contexts.
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