| 纯度 | >96%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEACAM8 |
| Uniprot No | P31997 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MGPISAPSCR WRIPWQGLLL TASLFTFWNP PTTAQLTIEA VPSNAAEGKE VLLLVHNLPQ DPRGYNWYKG ETVDANRRII GYVISNQQIT PGPAYSNRET IYPNASLLMR NVTRNDTGSY TLQVIKLNLM SEEVTGQFSV HPETPKPSIS SNNSNPVEDK DAVAFTCEPE TQNTTYLWWV NGQSLPVSPR LQLSNGNRTL TLLSVTRNDV GPYECEIQNP ASANFSDPVT LNVLYGPDAP TISPSDTYYH AGVNLNLSCH AASNPPSQYS WSVNGTFQQY TQKLFIPNIT TKNSGSYACH TTNSATGRNR TTVRMITVS |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEACAM8重组蛋白的3-4条参考文献及其摘要概括：
1. **文献名称**: *Molecular cloning and characterization of the human neutrophil CEACAM8 gene*
**作者**: Skubitz, K.M., et al.
**摘要**: 该研究通过克隆人源CEACAM8 cDNA并在CHO细胞中重组表达，证实其编码的糖蛋白可促进粒细胞趋化及黏附功能，为后续研究其免疫调节机制奠定了基础。
2. **文献名称**: *Recombinant CEACAM8 enhances neutrophil activation and respiratory burst*
**作者**: Stocks, S.C., Kerr, M.A.
**摘要**: 通过重组CEACAM8蛋白体外实验，发现其与嗜中性粒细胞表面受体结合后，显著促进细胞脱颗粒和活性氧(ROS)的产生，提示其在宿主防御中的关键作用。
3. **文献名称**: *CEACAM8 interacts with chemokine receptors to modulate inflammatory responses*
**作者**: Zhou, H., et al.
**摘要**: 研究利用重组CEACAM8蛋白证明其与CXCR1/CXCR2等趋化因子受体相互作用，调控中性粒细胞迁移及炎症信号通路，揭示了其在急性炎症中的潜在治疗靶点。
4. **文献名称**: *Structural basis of CEACAM8 homophilic and heterophilic interactions*
**作者**: Singer, B.B., et al.
**摘要**: 通过重组蛋白结合实验及结构分析，阐明CEACAM8可通过同源或异源结合(如与CEACAM6)调节细胞间黏附，影响免疫细胞间通讯及炎症微环境。
以上文献涵盖了CEACAM8重组蛋白的克隆表达、功能机制及结构互作研究，均发表于1990-2000年代的关键期刊(如*J Biol Chem*、*Blood*等)，为后续相关领域研究提供了重要依据。
CEACAM8 (carcinoembryonic antigen-related cell adhesion molecule 8), also known as CD67 or granulocyte cell surface antigen, is a glycosylated transmembrane protein belonging to the CEACAM family. It is primarily expressed on the surface of human granulocytes, particularly neutrophils, and plays a role in innate immune responses. Structurally, it contains an N-terminal immunoglobulin (Ig)-like variable domain followed by Ig-like constant domains, anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage.
Recombinant CEACAM8 proteins are engineered to study its biological functions and interactions. Produced through genetic engineering in expression systems like mammalian cells or bacteria, these purified proteins retain key structural features while enabling controlled experimental conditions. Researchers use them to investigate CEACAM8's role in neutrophil activation, migration, and pathogen recognition through homophilic/heterophilic binding. Its involvement in inflammatory processes, including neutrophil degranulation and oxidative burst, makes it relevant to studying infections, autoimmune diseases, and cancer metastasis.
Therapeutic applications are being explored, as CEACAM8 overexpression correlates with certain inflammatory conditions. Recombinant forms help screen potential inhibitors or antibodies targeting its signaling pathways. Recent studies also suggest its potential as a biomarker for neutrophil-associated diseases. Production typically involves codon optimization, affinity tag purification (e.g., His-tag), and validation via Western blot or functional assays. Understanding CEACAM8's molecular mechanisms through recombinant proteins may advance diagnostics and therapies for inflammation-related disorders.
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