| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACAT2 |
| Uniprot No | Q9BWD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTDSILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACAT2重组蛋白的3篇参考文献示例(注：以下文献为模拟示例，实际引用时请核实真实来源)：
---
1. **文献名称**: "Recombinant ACAT2 Expression in Mammalian Cells: Enzymatic Characterization and Role in Cholesterol Esterification"
**作者**: Chang, T.Y., et al.
**摘要**: 本研究在哺乳动物细胞中成功表达了重组ACAT2蛋白，分析了其酶动力学特性，证实ACAT2在肝脏和小肠中特异性催化胆固醇酯合成，为动脉粥样硬化研究提供模型。
2. **文献名称**: "Distinct Roles of ACAT1 and ACAT2 in Cellular Cholesterol Homeostasis Revealed by Recombinant Protein Studies"
**作者**: Anderson, R.A., et al.
**摘要**: 通过比较重组ACAT1和ACAT2的活性，发现ACAT2优先利用特定脂质底物，并参与血浆脂蛋白的胆固醇酯生成，揭示了两种同工酶在代谢中的功能差异。
3. **文献名称**: "Crystallographic Analysis of Recombinant ACAT2: Insights into Substrate Binding and Catalytic Mechanism"
**作者**: Guo, Z., et al.
**摘要**: 首次报道ACAT2重组蛋白的晶体结构，阐明其底物结合口袋及催化残基，为设计选择性抑制剂治疗高胆固醇血症提供结构基础。
---
如需真实文献，建议通过PubMed或Web of Science检索关键词“ACAT2 recombinant”或“ACAT2 expression”，筛选近年高引研究。
**Background of ACAT2 Recombinant Protein**
Acyl-CoA:cholesterol acyltransferase 2 (ACAT2) is a key enzyme in lipid metabolism, primarily responsible for esterifying free cholesterol with fatty acids to form cholesteryl esters. These esters are packaged into lipoproteins (e.g., VLDL and chylomicrons) for systemic transport. Unlike its isoform ACAT1. which is ubiquitously expressed and involved in cellular cholesterol homeostasis, ACAT2 is predominantly expressed in the liver and intestine, playing a critical role in dietary cholesterol absorption and hepatic lipoprotein secretion.
The recombinant ACAT2 protein is engineered for research to study its structure, enzymatic activity, and regulatory mechanisms. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant protein retains functional properties, enabling *in vitro* studies on cholesterol esterification kinetics and inhibitor screening. Its production has facilitated the development of assays to explore ACAT2's role in atherosclerosis, as excessive cholesteryl ester accumulation in arterial walls is a hallmark of the disease.
Research highlights ACAT2 as a potential therapeutic target for cardiovascular diseases. Pharmacological inhibition or genetic deletion of ACAT2 in animal models reduces atherogenesis without severely disrupting essential cholesterol metabolism, unlike broad ACAT inhibition. Recombinant ACAT2 also aids in structural studies, revealing substrate-binding domains and guiding the design of isoform-specific inhibitors. Current challenges include optimizing enzyme stability for crystallography and clarifying its interplay with lipid droplet proteins.
Overall, ACAT2 recombinant protein serves as a vital tool for deciphering its physiological and pathological roles, bridging gaps between biochemical insights and clinical applications in metabolic disorders.
×
