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| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | CXCL15 |
| Uniprot No | Q9WVL7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-167aa |
| 氨基酸序列 | QELRCLCIQEHSEFIPLKLIKNIMVIFETIYCNRKEVIAVPKNGSMICLDPDAPWVKATVGPITNRFLPEDLKQKEFPPAMKLLYSVEHEKPLYLSFGRPENKRIFPFPIRETSRHFADLAHNSDRNFLRDSSEVSLTGSDA |
| 预测分子量 | 23.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CXCL15重组蛋白的3篇代表性文献的简要总结：
1. **文献名称**: *"Recombinant mouse CXCL15 regulates neutrophil migration and suppresses inflammation in vivo"*
**作者**: Li Y. et al.
**摘要**: 该研究成功在大肠杆菌中表达并纯化了小鼠重组CXCL15蛋白，证实其能够通过激活CXCR2受体招募中性粒细胞，并在小鼠急性肺炎模型中抑制过度炎症反应，表明其在炎症调控中的潜在应用价值。
2. **文献名称**: *"Expression and functional characterization of human CXCL15 in non-small cell lung cancer"*
**作者**: Wang Q. et al.
**摘要**: 研究者利用哺乳动物细胞系统表达人源重组CXCL15.发现其能促进肺癌细胞迁移和血管生成，并通过激活PI3K/AKT信号通路增强肿瘤微环境中免疫细胞浸润，提示CXCL15在肿瘤进展中的作用机制。
3. **文献名称**: *"Bacterial endotoxin induces CXCL15 expression through TLR4/NF-κB pathway in alveolar epithelial cells"*
**作者**: Smith J.R. et al.
**摘要**: 该研究通过体外实验发现，脂多糖(LPS)刺激肺泡上皮细胞后，内源性CXCL15表达显著上调。进一步使用重组CXCL15蛋白验证其可增强中性粒细胞的趋化活性，为肺部感染中CXCL15的调控机制提供了实验依据。
注：上述文献为示例性内容，实际引用时请核对真实存在的文献。
CXCL15. a member of the CXC chemokine family, is a small secreted protein primarily involved in immune cell recruitment and inflammatory responses. It binds to the G protein-coupled receptor CXCR2. facilitating the migration of neutrophils and other leukocytes to sites of injury or infection. In humans, CXCL15 (originally named "lungkine") shares functional homology with mouse LIX or NCIL-1. though its expression profile differs across species. It is constitutively expressed in epithelial tissues, particularly the lung, gastrointestinal tract, and reproductive organs, playing roles in tissue homeostasis and pathogen defense.
Recombinant CXCL15 protein is engineered using bacterial (e.g., *E. coli*) or mammalian expression systems, often with tags like His or GST for purification. Its production enables researchers to study chemotaxis mechanisms, immune cell interactions, and signaling pathways *in vitro* or *in vivo*. Structurally, it contains the conserved ELR motif common to angiogenic CXC chemokines, though its functional duality—promoting both tissue repair and disease progression—has sparked interest in cancer and chronic inflammation research. In tumor microenvironments, CXCL15 may enhance immunosuppression by recruiting myeloid-derived suppressor cells, while in acute inflammation, it supports pathogen clearance.
Current applications include modeling neutrophil trafficking, exploring its role in diseases like colitis or lung injury, and evaluating therapeutic targeting of CXCR2-mediated pathways. However, its exact regulatory mechanisms and species-specific functions remain under investigation, highlighting the importance of recombinant protein tools in elucidating its complex biology.
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