| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPESP1 |
| Uniprot No | Q6UW49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-350aa |
| 氨基酸序列 | Y PSITVTPDEE QNLNHYIQVL ENLVRSVPSG EPGREKKSNS PKHVYSIASK GSKFKELVTH GDASTENDVL TNPISEETTT FPTGGFTPEI GKKKHTESTP FWSIKPNNVS IVLHAEEPYI ENEEPEPEPE PAAKQTEAPR MLPVVTESST SPYVTSYKSP VTTLDKSTGI GISTESEDVP QLSGETAIEK PEEFGKHPES WNNDDILKKI LDINSQVQQA LLSDTSNPAY REDIEASKDH LKRSLALAAA AEHKLKTMYK SQLLPVGRTS NKIDDIETVI NMLCNSRSKL YEYLDIKCVP PEMREKAATV FNTLKNMCRS RRVTALLKVY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPESP1重组蛋白的模拟参考文献示例(注：部分文献信息为假设，供参考)：
1. **文献名称**: "Recombinant SPESP1 facilitates sperm-egg fusion by interacting with egg membrane proteins"
**作者**: Tanaka K, et al.
**摘要**: 研究通过在大肠杆菌中表达重组SPESP1蛋白，发现其能特异性结合卵子透明带蛋白ZP2.并在体外受精实验中显著增强精子穿透卵子的能力，提示其在受精过程中的关键作用。
2. **文献名称**: "Structural and functional characterization of human SPESP1 recombinant protein"
**作者**: Smith JL, et al.
**摘要**: 利用昆虫细胞系统表达并纯化SPESP1重组蛋白，通过晶体学解析其三维结构，揭示其N端结构域具有结合精卵识别相关受体的功能位点，为不育症治疗提供新靶点。
3. **文献名称**: "SPESP1 knockout mice exhibit impaired fertility rescued by recombinant protein supplementation"
**作者**: Chen H, et al.
**摘要**: 构建SPESP1基因敲除小鼠模型，发现雄性不育表型;通过注射重组SPESP1蛋白可部分恢复精子功能，证实该蛋白在精子成熟中的必要性。
4. **文献名称**: "Proteomic analysis of SPESP1-interacting proteins using recombinant bait"
**作者**: Gupta R, et al.
**摘要**: 采用重组SPESP1蛋白进行pull-down实验，鉴定出多个与精子运动相关的互作蛋白(如ADAM家族成员)，探讨其调控精子功能的分子机制。
**提示**：实际文献需通过PubMed/Google Scholar检索确认，以上为基于SPESP1已知功能的模拟概括。
**Background of SPESP1 Recombinant Protein**
SPESP1 (Sperm Equatorin-Related Protein 1) is a testis-specific protein implicated in mammalian fertilization, particularly during sperm-egg membrane fusion. Initially identified in mice, SPESP1 localizes to the equatorial segment of sperm heads post-acrosome reaction, a critical step enabling sperm to penetrate the zona pellucida and bind the oocyte membrane. Studies suggest SPESP1 interacts with egg surface components, such as integrins or CD9 tetraspanin complexes, facilitating gamete adhesion or fusion. Its absence in knockout models leads to reduced fertility, underscoring its role in reproductive success.
Recombinant SPESP1 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, functional protein for mechanistic studies. This allows researchers to dissect its structural domains (e.g., transmembrane regions, glycosylation sites) and binding partners. Applications include *in vitro* fertilization assays, antibody development for fertility diagnostics, and exploring SPESP1's involvement in idiopathic infertility or contraceptive strategies.
Recent research also highlights potential roles beyond fertilization, such as in early embryonic development, though these remain less characterized. By leveraging recombinant SPESP1. scientists aim to unravel its molecular interactions and contribute to advancing reproductive medicine or novel therapies for infertility.
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