| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein phosphatase 4 regulatory subunit 3B, SMEK homolog 2, SMEK2, KIAA1387, PP4R3B, PPP4R3B |
| Entrez GeneID | 57223 |
| WB Predicted band size | 97.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SMEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-748 amino acids from the C-terminal region of human SMEK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SMEK2抗体的参考文献示例(注：部分内容基于文献主题的合理推断，建议通过学术数据库进一步验证)：
1. **文献名称**: "SMEK2/PP4R3B is a critical regulator of the DNA damage response"
**作者**: Lee JH, et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，利用SMEK2特异性抗体，揭示了SMEK2在调控DNA损伤应答中的核心作用，证明其缺失导致ATM/ATR信号通路异常。
2. **文献名称**: "Development and characterization of a monoclonal antibody specific for human SMEK2 protein"
**作者**: Zhang Y, et al.
**摘要**: 该文献报道了一种新型SMEK2单克隆抗体的开发，验证其特异性及在流式细胞术和免疫组化中的应用，为癌症中SMEK2表达分析提供工具。
3. **文献名称**: "SMEK2 regulates mitotic progression via PP4 phosphatase complex"
**作者**: Tanaka K, et al.
**摘要**: 研究使用SMEK2抗体进行共免疫沉淀和敲低实验，发现SMEK2通过PP4复合物调控纺锤体组装，影响细胞周期中的有丝分裂进程。
4. **文献名称**: "Functional interplay between SMEK1 and SMEK2 in oxidative stress response"
**作者**: Chen L, et al.
**摘要**: 通过siRNA和SMEK2抗体介导的蛋白检测，本文阐明SMEK2与同源蛋白SMEK1协同调节氧化应激下的细胞存活，提示其在神经退行性疾病中的潜在作用。
**建议**：以上文献标题和内容为示例性质，实际引用时请通过PubMed或Google Scholar以“SMEK2 antibody”、“PP4R3B”等关键词检索最新研究，并关注高影响力期刊(如*Cell Research*、*Journal of Biological Chemistry*)的相关论文。
The SMEK2 antibody is designed to detect SMEK2 (Suppressor of Mek null 2), also known as PP4R2. a regulatory subunit of protein phosphatase 4 (PP4). SMEK2 forms a complex with the catalytic subunit PP4C, modulating its phosphatase activity. This Ser/Thr phosphatase plays critical roles in diverse cellular processes, including DNA damage response, cell cycle regulation, apoptosis, and immune signaling. SMEK2. along with its homolog SMEK1. stabilizes PP4C and directs substrate specificity. Studies suggest SMEK2 is involved in dephosphorylating key targets like γH2AX during DNA repair and regulating T-cell receptor signaling.
The antibody, typically produced in rabbits or mice as monoclonal or polyclonal forms, is used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study SMEK2 expression, localization, and interactions. Its development has advanced research into PP4's regulatory mechanisms, particularly in cancer, neurodegeneration, and immune disorders. For instance, altered SMEK2 expression has been linked to tumor progression and chemoresistance. Validation often includes knockout cell lines to confirm specificity. As PP4 pathways gain therapeutic interest, the SMEK2 antibody remains a vital tool for dissecting phosphatase-related signaling networks.
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