| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP5 |
| Uniprot No | P45974 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-858aa |
| 氨基酸序列 | AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFL GFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAI GVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAV EALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSK CDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTI TPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMN QRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQ RKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGER VPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC RSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEE LLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQ AKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQ LRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPT SPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDF ANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNS LERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKY QLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIY FYQRVAS |
| 预测分子量 | 96 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USP5重组蛋白的3篇代表性文献示例(注：内容为模拟概括，具体文献需根据实际数据库检索确认)：
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1. **文献名称**：*Structural basis for ubiquitin recognition and cleavage by USP5*
**作者**：Li, Z., et al.
**摘要**：该研究解析了人源USP5重组蛋白的晶体结构，揭示了其特异性识别和切割线性泛素链的分子机制。通过体外酶活实验证明，重组USP5对K48和K63连接的泛素链具有去泛素化活性，为靶向USP5的药物设计提供结构基础。
2. **文献名称**：*USP5 regulates DNA damage repair through deubiquitinating BRCA1*
**作者**：Chen, Y., et al.
**摘要**：文章利用重组USP5蛋白进行功能研究，发现其通过去泛素化BRCA1调控DNA损伤修复通路。实验表明，USP5重组蛋白在体外可有效移除BRCA1上的泛素修饰，增强同源重组修复效率，提示其在癌症治疗中的潜在价值。
3. **文献名称**：*USP5 modulates antiviral immune response by stabilizing viral RNA sensor RIG-I*
**作者**：Wang, Q., et al.
**摘要**：本研究通过重组USP5蛋白与RIG-I的体外互作实验，证明USP5通过去泛素化稳定RIG-I蛋白，增强宿主抗病毒免疫应答。研究为病毒感染相关疾病的治疗策略提供了新视角。
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建议通过PubMed或Web of Science以“USP5 recombinant”为关键词检索最新文献，或关注领域内权威期刊如*Nature Communications*、*Cell Reports*等。如需具体文献DOI或全文，可进一步提供检索支持。
**Background of USP5 Recombinant Protein**
Ubiquitin-specific protease 5 (USP5), also known as isopeptidase T, is a member of the deubiquitinating enzyme (DUB) family, which regulates protein stability and function by removing ubiquitin moieties from target proteins. Ubiquitination, a post-translational modification, typically marks proteins for proteasomal degradation or modulates their activity, localization, or interactions. USP5 specifically cleaves unanchored ubiquitin chains, recycling free ubiquitin to maintain cellular ubiquitin homeostasis and prevent nonspecific degradation signals.
Structurally, USP5 contains a catalytic domain with conserved cysteine and histidine residues critical for its enzymatic activity, as well as ubiquitin-binding domains (UBDs) that recognize and process polyubiquitin chains. Its unique ability to hydrolyze unanchored ubiquitin chains distinguishes it from other DUBs, which often target substrate-linked ubiquitin.
Recombinant USP5 protein is produced in vitro using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag). This engineered protein retains the enzymatic activity of native USP5. enabling researchers to study its biochemical properties, substrate specificity, and regulatory mechanisms.
USP5 has garnered attention in biomedical research due to its role in diseases linked to ubiquitin dysregulation, such as cancer and neurodegenerative disorders. Overexpression of USP5 is observed in certain cancers, where it may promote tumor survival by stabilizing oncoproteins. Conversely, impaired USP5 activity has been associated with neuropathologies characterized by protein aggregation. Recombinant USP5 is widely used in drug discovery to screen for DUB inhibitors and to elucidate structural insights through techniques like X-ray crystallography. Its applications extend to in vitro assays exploring ubiquitin chain editing and crosstalk with other post-translational modifications.
In summary, USP5 recombinant protein serves as a vital tool for dissecting ubiquitin signaling pathways and developing therapeutic strategies targeting DUB-related diseases.
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