| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPA33 |
| Uniprot No | Q99795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-235aa |
| 氨基酸序列 | ISVETPQDVLRASQGKSVTLPCTYHTSTSSREGLIQWDKLLLTHTERVVI WPFSNKNYIHGELYKNRVSISNNAEQSDASITIDQLTMADNGTYECSVSL MSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTP QYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQF CNITVAVRSPSMNVVDHHHHHH |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPA33重组蛋白的3篇参考文献的简要信息(注：部分内容基于研究领域常见方向推断，建议通过学术数据库验证准确性)：
1. **文献名称**：*"Characterization of GPA33 as a novel target for antibody-based cancer immunotherapy"*
**作者**：Ritter G., et al.
**摘要**：研究报道了GPA33重组蛋白在结肠癌细胞表面的高表达特性，通过重组蛋白免疫小鼠成功制备单克隆抗体，证实其在肿瘤靶向治疗中的潜在应用。
2. **文献名称**：*"Structural and functional analysis of the GPA33 antigen using recombinant protein domains"*
**作者**：Heath J.K., et al.
**摘要**：利用重组GPA33蛋白解析其IgV样结构域的三维结构，揭示其与抗体结合的特定表位，为设计靶向药物提供结构基础。
3. **文献名称**：*"GPA33-directed CAR-T cells exhibit anti-tumor activity in colorectal cancer models"*
**作者**：Wang Y., et al.
**摘要**：研究基于GPA33重组蛋白筛选获得的高亲和力抗体构建CAR-T细胞，在动物模型中显著抑制结肠癌生长，验证其免疫治疗潜力。
**注意事项**：以上文献标题及摘要内容为领域内典型研究方向示例，实际文献可能存在差异，建议通过PubMed或Web of Science以“GPA33 recombinant”为关键词检索最新原文。
GPA33 (Glycoprotein A33) is a cell surface antigen belonging to the immunoglobulin superfamily, predominantly expressed in normal human gastrointestinal epithelial cells and overexpressed in colorectal cancer (CRC) tissues. It was first identified in the 1990s as a 43-48 kDa transmembrane glycoprotein with a unique structural organization: an extracellular domain containing two immunoglobulin-like folds (V-type and C2-type), a single-pass transmembrane region, and a short cytoplasmic tail. Its physiological role involves regulating cell-cell adhesion and maintaining epithelial barrier integrity through homophilic or heterophilic interactions.
The recombinant GPA33 protein, engineered via genetic cloning and expression in mammalian or bacterial systems, retains key functional domains for research and therapeutic applications. Its overexpression in >95% of CRC cases, coupled with limited expression in normal tissues, positions it as a promising biomarker and therapeutic target. Recombinant GPA33 enables studies on tumor biology, particularly in understanding CRC progression, metastasis, and immune evasion mechanisms.
In immunotherapy development, recombinant GPA33 has been utilized to generate monoclonal antibodies and antibody-drug conjugates (ADCs). Preclinical studies demonstrate that anti-GPA33 ADCs selectively deliver cytotoxic agents to CRC cells while sparing healthy tissues. Additionally, its role in modulating Wnt/β-catenin signaling pathways has sparked interest in combination therapies targeting CRC stem cells. Recent advances include bispecific antibodies engaging T-cells against GPA33-positive tumors and CAR-T cell engineering. Despite challenges like antigen heterogeneity, GPA33 remains a focal point in gastrointestinal oncology research due to its tumor-specific expression profile and functional relevance in carcinogenesis.
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