| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNTL1 |
| Uniprot No | Q8N428 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MRKIRANAIAILTVAWILGTFYYLWQDNRAHAASSGGRGAQRAGRRSEQLREDRTIPLIVTGTPSKGFDEKAYLSAKQLKAGEDPYRQHAFNQLESDKLSPDRPIRDTRHYSCPSVSYSSDLPATSVIITFHNEARSTLLRTVKSVLNRTPANLIQEIILVDDFSSDPEDCLLLTRIPKVKCLRNDRREGLIRSRVRGADVAAATVLTFLDSHCEVNTEWLPPMLQRVKEDHTRVVSPIIDVISLDNFAYLAASADLRGGFDWSLHFKWEQIPLEQKMTRTDPTRPIRTPVIAGGIFVIDKSWFNHLGKYDAQMDIWGGENFELSFRVWMCGGSLEIVPCSRVGHVFRKRHPYNFPEGNALTYIRNTKRTAEVWMDEYKQYYYEARPSAIGKAFGSVATRIEQRKKMNCKSFRWYLENVYPELTVPVKEALPGIIKQGVNCLESQGQNTAGDFLLGMGICRGSAKNPQPAQAWLFSDHLIQQQGKCLAATSTLMSSPGSPVILQMCNPREGKQKWRRKGSFIQHSVSGLCLETKPAQLVTSKCQADAQAQQWQLLPHT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GALNTL1重组蛋白的3篇示例参考文献(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**: *Expression and Functional Analysis of Recombinant GALNTL1 in Sperm Motility Regulation*
**作者**: Zhang, L.; Wang, Q.; Chen, H.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了GALNTL1重组蛋白，发现其通过调控糖基化修饰影响精子运动能力，为男性不育症机制研究提供了新方向。
2. **文献名称**: *Structural Characterization of GALNTL1 Recombinant Protein and Its Glycosyltransferase Activity*
**作者**: Smith, J.R.; Tanaka, K.; Lee, S.
**摘要**: 通过X射线晶体学解析GALNTL1重组蛋白的三维结构，揭示了其催化结构域的关键氨基酸位点，并证实其对特定底物的GalNAc转移酶活性。
3. **文献名称**: *GALNTL1 Recombinant Protein Enhances O-Linked Glycosylation in vitro*
**作者**: Guo, X.; Liu, Y.; Zhou, M.
**摘要**: 利用哺乳动物细胞系统表达GALNTL1重组蛋白，证明其能显著提高细胞外基质蛋白的O-糖基化水平，暗示其在组织发育中的潜在作用。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“GALNTL1 recombinant protein”获取最新研究。
**Background of GALNTL1 Recombinant Protein**
GALNTL1 (polypeptide N-acetylgalactosaminyltransferase-like protein 1) is a member of the glycosyltransferase family, which plays a critical role in post-translational protein modification. This enzyme is involved in initiating O-linked glycosylation, a process where sugar molecules (GalNAc) are attached to serine or threonine residues of target proteins. Such modifications influence protein stability, trafficking, and interactions, impacting cellular signaling, immunity, and tissue development.
The *GALNTL1* gene, located on human chromosome 14. is expressed in specific tissues, including the testis, brain, and respiratory epithelium. Studies suggest its potential role in spermatogenesis, neurodevelopment, and mucin production. Dysregulation of GALNTL1 has been loosely associated with reproductive disorders and certain cancers, though its precise mechanistic contributions remain under investigation.
Recombinant GALNTL1 protein is produced using heterologous expression systems (e.g., *E. coli*, HEK293. or insect cells) to enable functional studies. Purified recombinant protein facilitates *in vitro* assays to characterize enzymatic activity, substrate specificity, and interactions with other glycoproteins or inhibitors. It also aids in antibody development for diagnostic or therapeutic research.
Current research focuses on elucidating GALNTL1's biological significance, particularly in fertility and disease contexts. Its recombinant form serves as a tool to explore glycosylation pathways and identify novel biomarkers or therapeutic targets. However, challenges persist in defining its full range of substrates and regulatory mechanisms, highlighting the need for further structural and functional studies.
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