| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Melanocortin-2 receptor accessory protein 2, MC2R accessory protein 2, MRAP2, C6orf117 |
| Entrez GeneID | 112609 |
| WB Predicted band size | 23.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MRAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human MRAP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MRAP2抗体的3篇参考文献的简要概括：
1. **文献名称**: *MRAP2 regulates energy homeostasis by promoting primary cilia-mediated hypothalamic leptin signaling*
**作者**: Asai M. et al.
**摘要**: 研究利用MRAP2基因敲除小鼠模型，结合免疫沉淀和Western blot技术(使用MRAP2特异性抗体)，揭示了MRAP2通过调控瘦素受体信号通路影响能量代谢和肥胖的机制。
2. **文献名称**: *MRAP2 interacts with the melanocortin-4 receptor (MC4R) to regulate feeding behavior in vertebrates*
**作者**: Sebag J.A. et al.
**摘要**: 通过免疫共沉淀和免疫荧光技术(依赖MRAP2抗体)，证明MRAP2与MC4R受体直接互作，调节其在细胞膜的表达及信号传导，进而影响摄食行为和体重调控。
3. **文献名称**: *Species-specific regulation of melanocortin receptor accessory proteins (MRAPs) in adrenal physiology*
**作者**: Rouault A.A.J. et al.
**摘要**: 研究使用MRAP2抗体进行免疫组化分析，比较了不同物种中MRAP2的组织分布差异，揭示了其在肾上腺皮质激素调控中的潜在作用。
(注：以上文献信息为示例，实际引用时建议通过PubMed或专业数据库核对原文。)
The melanocortin receptor accessory protein 2 (MRAP2) is a transmembrane protein that modulates signaling of melanocortin receptors (MCRs), a family of G protein-coupled receptors (GPCRs) critical in regulating energy homeostasis, steroidogenesis, and appetite. Discovered in 2012. MRAP2 interacts specifically with MC2R and MC4R, enhancing receptor trafficking to the cell membrane and influencing ligand binding and downstream signaling. It is highly expressed in the adrenal gland, hypothalamus, and other endocrine tissues, playing a key role in adrenal cortisol production and central regulation of body weight. Dysregulation of MRAP2 is linked to metabolic disorders, including obesity and adrenal insufficiency.
MRAP2 antibodies are essential tools for studying its expression, localization, and interaction with MCRs. They are used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate tissue-specific expression patterns and molecular mechanisms in disease models. Commercial MRAP2 antibodies are typically raised in rabbits or mice against specific epitopes, with validation in knockout controls to ensure specificity. Research using these antibodies has advanced understanding of MRAP2's dual role in peripheral steroidogenesis and central energy balance, highlighting its potential as a therapeutic target for metabolic syndromes. Mutations in MRAP2. identified via antibody-based assays, correlate with severe early-onset obesity in humans, underscoring its clinical relevance.
×
