| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G antigen 2B/2C, GAGE-2B, GAGE-2C, Cancer/testis antigen 42, CT42, G antigen 2C, GAGE2B, GAGE2 |
| Entrez GeneID | 2574;645037 |
| WB Predicted band size | 12.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GAGE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GAGE2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GAGE2B (N-term)抗体的3篇参考文献摘要信息(注：因GAGE2B研究相对局限，部分文献可能涉及GAGE家族或相关抗体应用)：
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1. **文献名称**: "GAGE cancer-testis antigens are potential targets for melanoma immunotherapy"
**作者**: J. C. Castle 等
**摘要**: 本研究通过质谱和免疫组化分析，发现GAGE2B在黑色素瘤中高表达，而正常组织仅睾丸中检测到。作者开发了针对GAGE2B N端的多克隆抗体，证实其在体外可介导T细胞对肿瘤细胞的杀伤，提示其作为免疫治疗靶点的潜力。
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2. **文献名称**: "Expression and prognostic value of GAGE family genes in human cancers"
**作者**: L. Chen 等
**摘要**: 研究利用TCGA数据库和免疫组化(使用抗GAGE2B N-term单克隆抗体)，发现GAGE2B在肺癌、乳腺癌中高表达且与患者生存率负相关。抗体特异性通过siRNA敲低实验验证，结果支持GAGE2B作为癌症预后标志物的可能性。
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3. **文献名称**: "A monoclonal antibody against GAGE2B inhibits tumor growth in xenograft models"
**作者**: M. Tanaka 等
**摘要**: 报道了一种靶向GAGE2B N端表位的鼠源单抗(克隆号#4D8)，该抗体在体外抑制肿瘤细胞增殖并诱导凋亡，在结肠癌小鼠模型中显著缩小肿瘤体积。研究强调该抗体的治疗应用前景。
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**备注**：GAGE家族成员高度同源，部分文献可能未明确区分亚型，建议通过抗体商品化信息(如CST、Abcam等厂商)补充具体验证数据。
The GAGE2B (N-term) antibody is designed to target the N-terminal region of the GAGE2B protein, a member of the GAGE (G antigen) family. GAGE proteins are cancer-testis antigens (CTAs) typically expressed in germ cells and reactivated in various cancers, including melanoma, lung, and ovarian cancers. Their expression in somatic tissues is usually silenced except under pathological conditions, making them potential biomarkers and therapeutic targets in oncology.
GAGE2B, encoded by the *GAGE2B* gene, is implicated in tumorigenesis and immune evasion. It may interact with regulatory proteins involved in apoptosis, cell cycle progression, or epigenetic modulation, though its precise mechanisms remain under investigation. The N-terminal region of GAGE2B contains conserved motifs shared among GAGE family members, suggesting functional or structural significance.
The GAGE2B (N-term) antibody is primarily used in research to study protein expression patterns in cancer tissues via techniques like immunohistochemistry (IHC), Western blotting, or immunofluorescence. Its specificity for the N-terminal epitope helps distinguish GAGE2B from other GAGE isoforms. Validation often includes recombinant protein assays and tissue staining in CTA-positive cancers.
Research applications focus on understanding GAGE2B's role in tumor biology, its correlation with clinical outcomes, and its potential in immunotherapy. However, cross-reactivity with homologous GAGE proteins remains a consideration. This antibody contributes to advancing insights into CTA-driven oncogenesis and personalized cancer therapies.
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