| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINA6 |
| Uniprot No | P08185 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-405aa |
| 氨基酸序列 | MDPNAAYVNMSNHHRGLASANVDFAFSLYKHLVALSPKKNIFISPVSISMALAMLSLGTCGHTRAQLLQGLGFNLTERSETEIHQGFQHLHQLFAKSDTSLEMTMGNALFLDGSLELLESFSADIKHYYESEVLAMNFQDWATASRQINSYVKNKTQGKIVDLFSGLDSPAILVLVNYIFFKGTWTQPFDLASTREENFYVDETTVVKVPMMLQSSTISYLHDSELPCQLVQMNYVGNGTVFFILPDKGKMNTVIAALSRDTINRWSAGLTSSQVDLYIPKVTISGVYDLGDVLEEMGIADLFTNQANFSRITQDAQLKSSKVVHKAVLQLNEEGVDTAGSTGVTLNLTSKPIILRFNQPFIIMIFDHFTWSSLFLARVMNPV |
| 预测分子量 | 69.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINA6重组蛋白的3篇代表性文献及其摘要内容：
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1. **文献名称**：*Expression and characterization of recombinant human corticosteroid-binding globulin*
**作者**：Hammond, G.L., Smith, C.L., Goping, I.S., et al.
**摘要**：该研究首次报道了在哺乳动物细胞中重组表达人SERPINA6(CBG)蛋白的方法，验证了其与皮质醇的高亲和力结合能力，并分析了其糖基化修饰对蛋白稳定性和激素运输功能的影响。
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2. **文献名称**：*Structural insights into the pH-dependent conformational change of recombinant human CBG*
**作者**：Lin, H.Y., Mueller, J.W., Tran, N., et al.
**摘要**：通过X射线晶体学解析了重组SERPINA6蛋白的pH依赖性构象变化，揭示了其在不同生理环境下调控皮质醇释放的结构机制，为理解CBG在应激反应中的作用提供分子基础。
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3. **文献名称**：*Recombinant SERPINA6 as a potential biomarker for adrenal dysfunction*
**作者**：Zhang, Y., Wang, Q., Li, R., et al.
**摘要**：研究发现，重组SERPINA6蛋白在体外和动物模型中能灵敏反映皮质醇代谢异常，提出其作为肾上腺功能紊乱的新型诊断标志物，并验证了其与血清游离皮质醇水平的负相关性。
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**备注**：以上文献信息为基于领域研究的概括性示例，实际引用需通过PubMed或学术数据库检索具体论文。早期研究(如Hammond等)多关注重组表达技术，近年研究更侧重结构功能或临床应用。
SERPINA6. also known as corticosteroid-binding globulin (CBG), is a member of the serine protease inhibitor (SERPIN) superfamily. It is primarily synthesized in the liver and secreted into the bloodstream, where it functions as a transport protein for glucocorticoids, notably cortisol, and progesterone. By binding approximately 90% of circulating cortisol, SERPINA6 regulates the bioavailability of this critical stress hormone, influencing metabolic, immune, and inflammatory responses. Its role extends beyond passive transport; proteolytic cleavage of SERPINA6 by neutrophil elastase at inflammatory sites releases cortisol locally, modulating tissue-specific glucocorticoid activity.
Recombinant SERPINA6 protein is produced using biotechnological methods, often through expression in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, including glycosylation. This engineered protein retains the functional capacity to bind corticosteroids with high affinity, mimicking native SERPINA6. Researchers utilize it to study glucocorticoid distribution dynamics, investigate hormonal dysregulation in conditions like Cushing's syndrome or adrenal insufficiency, and explore its interplay with SERPINA1 (alpha-1 antitrypsin) in inflammation. Additionally, it serves as a critical reagent in developing diagnostic assays for cortisol quantification and drug discovery platforms targeting glucocorticoid-related pathways. Recent studies also probe its potential role in metabolic disorders and stress response mechanisms, highlighting its multifaceted biological significance.
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