| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CES1 |
| Uniprot No | P23141-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-562aa |
| 氨基酸序列 | GHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTP PQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCL YLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVT IQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTI FGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQ IAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQP LLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPL SEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFL DLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDH GDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWP EYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEVDHHH HHH |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CES1重组蛋白的3篇参考文献示例(部分信息为示例性描述，建议核实具体文献)：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of Recombinant Human Carboxylesterase 1 (CES1) in Insect Cells*"
**作者**: Wang et al.
**摘要**: 研究利用杆状病毒-昆虫细胞系统表达重组人CES1蛋白，验证其水解活性(如可卡因代谢)，并分析酶动力学特性，证明其可用于体外药物代谢研究。
2. **文献名称**: *"Optimization of Recombinant CES1 Production in Pichia pastoris for Pharmaceutical Applications"*
**作者**: Kim & Park
**摘要**: 通过毕赤酵母表达系统优化CES1重组蛋白的分泌表达，纯化后酶活性显著提高，为规模化生产用于药物代谢酶研究提供方法。
3. **文献名称**: *"Structural Insights into Substrate Specificity of Recombinant CES1 through Site-Directed Mutagenesis"*
**作者**: Thompson et al.
**摘要**: 通过定点突变和晶体结构分析，揭示重组CES1底物结合口袋的关键氨基酸残基，阐明其对酯类前药(如氯吡格雷)的选择性水解机制。
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**提示**：实际文献可通过PubMed或Google Scholar搜索关键词“recombinant CES1 expression”或“carboxylesterase 1 purification”获取。
Carboxylesterase 1 (CES1) is a key enzyme belonging to the serine hydrolase superfamily, primarily expressed in the liver, macrophages, and adipose tissue. It plays a critical role in hydrolyzing ester- or amide-containing compounds, including clinically relevant drugs (e.g., clopidogrel, methylphenidate), environmental toxins, and endogenous lipids. CES1-mediated metabolism influences drug efficacy, toxicity, and pharmacokinetics, making it a focal point in pharmacogenomics and personalized medicine.
The recombinant CES1 protein is engineered via heterologous expression systems (e.g., E. coli, mammalian cells) to study its structure-function relationships, substrate specificity, and regulatory mechanisms. Its recombinant form enables high-purity production for biochemical assays, inhibitor screening, and structural studies (e.g., X-ray crystallography). Notably, CES1 polymorphisms and altered activity are linked to metabolic disorders (e.g., hypercholesterolemia), neurotoxicity, and variable drug responses, driving demand for recombinant tools to explore these associations.
Recent research also highlights CES1's role in lipid homeostasis and viral infections (e.g., SARS-CoV-2 spike protein processing), expanding its therapeutic relevance. Advances in protein engineering, such as codon optimization and fusion tags, have enhanced recombinant CES1 stability and solubility, facilitating drug development and mechanistic studies. However, challenges remain in mimicking native post-translational modifications and tissue-specific isoforms.
Overall, recombinant CES1 serves as a vital resource for unraveling metabolic pathways, optimizing drug design, and addressing interindividual variability in treatment outcomes, underscoring its importance in both basic research and clinical applications.
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