| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA5A |
| Uniprot No | Q13591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-968aa |
| 氨基酸序列 | MKGTCVIAWL FSSLGLWRLA HPEAQGTTQC QRTEHPVISY KEIGPWLREF RAKNAVDFSQ LTFDPGQKEL VVGARNYLFR LQLEDLSLIQ AVEWECDEAT KKACYSKGKS KEECQNYIRV LLVGGDRLFT CGTNAFTPVC TNRSLSNLTE IHDQISGMAR CPYSPQHNST ALLTAGGELY AATAMDFPGR DPAIYRSLGI LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH DCGKTVFSRA ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQSTFFLPEL DLIYGIFTTN VNSIAASAVC VFNLSAIAQA FSGPFKYQEN SRSAWLPYPN PNPHFQCGTV DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS RFSHVAVDVV QGREALVHII YLATDYGTIK KVRVPLNQTS SSCLLEEIEL FPERRREPIR SLQILHSQSV LFVGLREHVV KIPLKRCQFY RTRSTCIGAQ DPYCGWDVVM KKCTSLEESL SMTQWEQSIS ACPTRNLTVD GHFGVWSPWT PCTHTDGSAV GSCLCRTRSC DSPAPQCGGW QCEGPGMEIA NCSRNGGWTP WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC PPHMFWTGWG PWERCTAQCG GGIQARRRIC ENGPDCAGCN VEYQSCNTNP CPELKKTTPW TPWTPVNISD NGGHYEQRFR YTCKARLADP NLLEVGRQRI EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA HTVNGAWSAW TSWSQCSRDC SRGIRNRKRV CNNPEPKYGG MPCLGPSLEY QECNILPCPV DGVWSCWSPW TKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQPCPESWS EWSDWSECEA SGVQVRARQC ILLFPMGSQC SGNTTESRPC VFDSNFIPEV SVARSSSVEE KRCGEFNM |
| 预测分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Semaphorin 5A suppresses angiogenesis by regulating endothelial cell migration and apoptosis"**
- *Author(s): Li X, et al.*
- **摘要**: 研究利用重组SEMA5A蛋白，证明其通过抑制内皮细胞迁移和诱导凋亡来抑制肿瘤血管生成，机制涉及整合素信号通路调控。
2. **"Recombinant SEMA5A regulates glioblastoma growth and invasion via plexin-B1 interactions"**
- *Author(s): Wang Y, et al.*
- **摘要**: 通过表达纯化的重组SEMA5A，发现其通过结合plexin-B1受体抑制胶质母细胞瘤细胞侵袭，并降低MMP-2/9活性。
3. **"SEMA5A as a diagnostic biomarker in colorectal cancer: Recombinant protein-based validation"**
- *Author(s): Chen L, et al.*
- **摘要**: 研究利用重组SEMA5A蛋白建立ELISA检测方法，证实其在结直肠癌患者血清中高表达，与转移和预后不良相关。
4. **"Structural characterization of recombinant SEMA5A and its role in neuronal development"**
- *Author(s): Parker M, et al.*
- **摘要**: 通过哺乳动物系统表达SEMA5A重组蛋白，解析其结构域功能，证明其通过排斥性信号调控神经元突触形成和轴突导向。
(注：以上为虚构文献示例，实际文献需通过PubMed/Google Scholar检索确认。)
SEMA5A (Semaphorin 5A) is a member of the semaphorin family, a group of secreted and membrane-associated proteins initially identified for their role in axonal guidance and neuronal development. As a class 5 semaphorin, SEMA5A features a conserved ~500-amino acid "sema domain" critical for receptor binding, followed by thrombospondin type-1 repeats (TSP1), and a transmembrane domain in its native form. However, recombinant SEMA5A proteins are typically engineered as soluble extracellular fragments (e.g., sema domain + TSP1 repeats) produced in mammalian or insect expression systems, often fused with tags like Fc or His for purification and detection.
Functionally, SEMA5A acts as a bifunctional guidance cue, modulating cell migration, angiogenesis, and tumor progression by interacting with receptors such as plexins and neuropilins. It exhibits context-dependent roles in cancer: in pancreatic cancer and glioblastoma, SEMA5A promotes tumor invasiveness and angiogenesis, whereas in colorectal cancer, it may suppress metastasis. Its dysregulation is also implicated in neurodevelopmental disorders, particularly autism spectrum disorder (ASD), where reduced SEMA5A expression correlates with impaired synaptic connectivity.
Recombinant SEMA5A is widely used to study these pathways in vitro or in animal models. Researchers employ it to dissect signaling mechanisms, screen for therapeutic modulators, or explore its potential as a biomarker. Its dual role in both promoting and inhibiting biological processes underscores the importance of context-specific studies, making recombinant SEMA5A a valuable tool for unraveling its complex pathophysiology in development and disease.
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