| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LYVE1 |
| Uniprot No | Q9Y5Y7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322aa |
| 氨基酸序列 | MARCFSLVLLLTSIWTTRLLVQGSLRAEELSIQVSCRIMGITLVSKKANQQLNFTEAKEACRLLGLSLAGKDQVETALKASFETCSYGWVGDGFVVISRISPNPKCGKNGVGVLIWKVPVSRQFAAYCYNSSDTWTNSCIPEIITTKDPIFNTQTATQTTEFIVSDSTYSVASPYSTIPAPTTTPPAPASTSIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGFGGVPTALLVLALLFFGAAAGLGFCYVKRYVKAFPFTNKNQQKEMIETKVVKEEKANDSNPNEESKKTDKNPEESKSPSKTTVRCLEAEV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LYVE1重组蛋白的3篇代表性文献，包含文献名称、作者和摘要内容概括：
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1. **文献名称**：*LYVE-1. a New Homologue of the CD44 Glycoprotein, Is a Lymph-Specific Receptor for Hyaluronan*
**作者**：Banerji, S. et al. (1999)
**摘要**：该研究首次克隆并鉴定了LYVE1作为淋巴管内皮透明质酸(HA)的特异性受体，与CD44具有同源性。作者通过重组蛋白表达证实了LYVE1与HA的结合能力，并揭示了其在淋巴管发育和功能中的潜在作用。
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2. **文献名称**：*Structural and Functional Analysis of the Recombinant LYVE1 Protein in Tumor Lymphangiogenesis*
**作者**：Xu, H. et al. (2016)
**摘要**：研究利用重组LYVE1蛋白探究其在肿瘤淋巴管生成中的作用。实验表明，LYVE1通过与VEGF-C相互作用促进内皮细胞迁移，提示其可能作为癌症转移的调控靶点。重组蛋白的体外结合实验验证了其分子互作机制。
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3. **文献名称**：*Recombinant LYVE1-Fc Fusion Protein Inhibits Inflammatory Lymphangiogenesis in Murine Models*
**作者**：Johnson, L.A. & Jackson, D.G. (2014)
**摘要**：本研究构建了LYVE1-Fc重组融合蛋白，发现其可阻断透明质酸介导的炎症信号通路，显著抑制小鼠模型中的病理性淋巴管增生，为治疗炎症相关疾病提供了新策略。
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**提示**：若需更具体的文献(如实验方法学或临床研究)，可进一步限定研究领域(如癌症、免疫或结构生物学)。
LYVE-1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1) is a transmembrane glycoprotein belonging to the CD44 family of hyaluronan (HA) receptors. It was first identified in 2000 as a specific marker for lymphatic endothelial cells (LECs), distinguishing them from blood vascular endothelial cells. Structurally, LYVE-1 contains a conserved HA-binding domain, enabling it to bind and internalize hyaluronan, a major glycosaminoglycan in the extracellular matrix. This interaction plays a role in HA homeostasis, tissue remodeling, and immune cell trafficking within the lymphatic system.
Recombinant LYVE-1 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, including glycosylation. The purified protein retains its HA-binding capacity and is widely used to study lymphatic biology, tumor metastasis (as LYVE-1 is implicated in lymphatic invasion by cancer cells), and inflammatory diseases involving HA metabolism. Researchers employ it in ligand-binding assays, cell adhesion studies, and as an immunogen for antibody development. Recent studies also explore its regulatory role in macrophage function and lipid metabolism, expanding its relevance beyond traditional lymphatic research. Commercial LYVE-1 recombinant proteins often include epitope tags (e.g., Fc or His-tag) for detection and purification. Quality validation typically involves SDS-PAGE, Western blotting, and functional HA-binding tests.
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