| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH16 |
| Uniprot No | O75309 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-829aa |
| 氨基酸序列 | MVPAWLWLLCVSVPQALPKAQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSPPGTEVTRLSAEDADAPGSPNSHVVYQLLSPEPEDGVEGRAFQVDPTSGSVTLGVLPLRAGQNILLLVLAMDLAGAEGGFSSTCEVEVAVTDINDHAPEFITSQIGPISLPEDVEPGTLVAMLTAIDADLEPAFRLMDFAIERGDTEGTFGLDWEPDSGHVRLRLCKNLSYEAAPSHEVVVVVQSVAKLVGPGPGPGATATVTVLVERVMPPPKLDQESYEASVPISAPAGSFLLTIQPSDPISRTLRFSLVNDSEGWLCIEKFSGEVHTAQSLQGAQPGDTYTVLVEAQDTDEPRLSASAPLVIHFLKAPPAPALTLAPVPSQYLCTPRQDHGLIVSGPSKDPDLASGHGPYSFTLGPNPTVQRDWRLQTLNGSHAYLTLALHWVEPREHIIPVVVSHNAQMWQLLVRVIVCRCNVEGQCMRKVGRMKGMPTKLSAVGILVGTLVAIGIFLILIFTHWTMSRKKDPDQPADSVPLKATV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH16重组蛋白的模拟参考文献示例(实际文献需通过学术数据库核实)：
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1. **《CDH16重组蛋白的制备及其在肾细胞癌中的表达分析》**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌表达系统成功纯化CDH16重组蛋白，并发现其在肾细胞癌组织中显著高表达，提示其可能作为肾癌诊断的潜在生物标志物。
2. **《Cadherin-16重组蛋白介导的细胞黏附功能研究》**
- 作者：Yamamoto K, et al.
- 摘要：利用CDH16重组蛋白验证其在肾小管上皮细胞中的同源黏附作用，证明其通过钙离子依赖性机制参与细胞间连接，影响组织极性维持。
3. **《CDH16重组蛋白与Wnt/β-catenin信号通路的相互作用》**
- 作者：Chen L, et al.
- 摘要：通过体外实验发现CDH16重组蛋白可通过调控β-catenin的核转位抑制Wnt通路活性，为探究其在肾脏发育和肿瘤发生中的作用提供依据。
4. **《重组CDH16在急性肾损伤模型中的保护效应》**
- 作者：Wang R, et al.
- 摘要：动物实验表明，外源性CDH16重组蛋白能减轻缺血再灌注诱导的肾小管损伤，机制可能与减少细胞凋亡和炎症反应相关。
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建议通过PubMed、Web of Science等平台检索真实文献，结合关键词“CDH16 recombinant protein”或“KSP-cadherin”获取最新研究。
CDH16 (Cadherin-16), also known as KSP-cadherin or kidney-specific cadherin, is a calcium-dependent cell adhesion protein belonging to the cadherin superfamily. It is primarily expressed in the epithelial cells of the kidney, particularly in the distal tubules and collecting ducts, and has limited detection in thyroid tissues. As a classical type II cadherin, CDH16 plays a critical role in maintaining tissue architecture and polarity through homophilic cell-cell adhesion. Its extracellular domain contains five cadherin repeats that mediate calcium-dependent intercellular interactions, while the intracellular domain interacts with catenins to link the actin cytoskeleton.
Recombinant CDH16 protein is engineered using molecular cloning techniques to express and purify the protein in vitro, often in mammalian or bacterial expression systems. This engineered version retains functional domains essential for adhesion and signaling studies. Researchers utilize recombinant CDH16 to investigate kidney development, epithelial barrier formation, and pathological processes such as renal fibrosis or tumorigenesis. Its tissue-specific expression profile makes it a potential biomarker for renal cell carcinoma and thyroid-related disorders.
Recent studies explore CDH16's role in Wnt/β-catenin signaling modulation and its interaction with other junctional proteins. Pharmaceutical applications include developing antibodies for diagnostic tools or therapeutic targeting in kidney diseases. However, functional studies remain challenging due to cadherin family redundancy and context-dependent signaling. Current research focuses on structural characterization of its adhesive interfaces and disease-associated mutations. Recombinant CDH16 serves as a vital tool for decoding cadherin-mediated mechanisms in organogenesis and epithelial pathophysiology.
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