| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BHMT2 |
| Uniprot No | Q9H2M3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAPAGRPGAKKGILERLESGEVVIGDG SFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFS ASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEAR IKKLFRQQLEVFAWKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMC IGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLE WAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAR EAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLD MHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BHMT2重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
1. **文献名称**: "Recombinant expression and functional characterization of human betaine-homocysteine S-methyltransferase 2 (BHMT2)"
**作者**: Smith JL, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了人源BHMT2蛋白，并通过体外酶活实验证实其催化同型半胱氨酸与甜菜碱转化为二甲基甘氨酸和甲硫氨酸的能力。研究还发现重组BHMT2的活性依赖于锌离子的结合，并揭示了其与BHMT1的底物特异性差异。
2. **文献名称**: "Crystal structure of BHMT2 reveals insights into substrate binding and catalytic mechanism"
**作者**: Chen H, et al.
**摘要**: 通过X射线晶体学解析了重组人源BHMT2的3D结构，明确了其与底物甜菜碱和同型半胱氨酸的结合位点。结构分析表明，BHMT2的活性中心构象与BHMT1存在显著差异，解释了二者在代谢调控中的功能分化。
3. **文献名称**: "Role of BHMT2 in hepatic one-carbon metabolism: Insights from recombinant protein studies"
**作者**: Gupta R, et al.
**摘要**: 利用重组BHMT2蛋白探究其在肝脏一碳代谢中的作用，发现BHMT2通过调节甲硫氨酸循环影响同型半胱氨酸水平。体外实验表明，BHMT2的表达水平与氧化应激条件下细胞的抗氧化能力相关，提示其在病理生理中的潜在作用。
注：以上文献信息为示例性质，实际文献需通过学术数据库检索确认。
**Background of BHMT2 Recombinant Protein**
Betaine-homocysteine S-methyltransferase 2 (BHMT2), also known as betaine—homocysteine methyltransferase-like protein, is a metabolic enzyme involved in the regulation of homocysteine and methionine metabolism. While its homolog BHMT1 is well-characterized in the liver and kidneys, catalyzing the conversion of homocysteine to methionine using betaine as a methyl donor, BHMT2 remains less studied. Emerging evidence suggests BHMT2 may play a complementary or tissue-specific role in maintaining one-carbon metabolism, particularly under conditions of folate deficiency or oxidative stress.
Structurally, BHMT2 shares sequence homology with BHMT1 but exhibits distinct substrate preferences and expression patterns. It is encoded by the *BHMT2* gene, located on human chromosome 12. and is expressed in tissues like the liver, brain, and placenta. Recombinant BHMT2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its enzymatic activity, substrate specificity, and interaction with metabolic pathways.
Research on BHMT2 has gained attention due to its potential link to diseases associated with homocysteine imbalance, such as cardiovascular disorders, neurodegenerative conditions, and pregnancy complications. Recombinant BHMT2 enables in vitro studies to dissect its role in redox regulation, DNA methylation, and cellular detoxification. Additionally, it serves as a tool for screening inhibitors or activators that could modulate homocysteine levels, offering therapeutic avenues.
Despite progress, the physiological significance of BHMT2 remains under investigation, with ongoing studies aimed at clarifying its metabolic contributions and regulatory mechanisms in health and disease.
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