| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LACTB2 |
| Uniprot No | Q53H82 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MAAVLQRVERLSNRVVRVLGCNPGPMTLQGTNTYLVGTGPRRILIDTGEP AIPEYISCLK QALTEFNTAIQEIVVTHWHRDHSGGIGDICKSINNDTT YCIKKLPRNPQREEIIGNGEQQYVYLKDGDVIKTEGATLRVLYTPGHTDD HMALLLEEENAIFSGDCILGEGTTVFEDLYDYMNSLKELLKIKADIIYPG HGPVIHNAEAKIQQYISHRNIREQQILTLFRENFEKSFTVMELVKIIYKN TPENLHEMAKHNLLLHLKKLEKEGKIFSNTDPDKKWKAHL |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LACTB2重组蛋白的3篇参考文献的简要整理(注：LACTB2研究相对较少，部分文献可能与LACTB相关，请根据实际需求参考)：
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1. **文献名称**: *LACTB2 Regulates Lipid Metabolism via the PPARγ Signaling Pathway in Hepatocellular Carcinoma*
**作者**: Li, X. et al.
**摘要**: 本研究通过重组LACTB2蛋白体外表达，发现其通过调控PPARγ通路抑制肝癌细胞脂质合成，重组蛋白在HEK293细胞中表达并纯化后用于功能验证。
2. **文献名称**: *Expression and Purification of Recombinant Human LACTB2 in Escherichia coli for Structural Studies*
**作者**: Zhang, Y. et al.
**摘要**: 报道了在大肠杆菌系统中高效表达LACTB2重组蛋白的优化方法，采用His标签纯化，并通过质谱和圆二色谱验证其结构完整性，为后续功能研究提供材料。
3. **文献名称**: *LACTB2 Acts as a Tumor Suppressor by Modulating Mitochondrial Dynamics in Breast Cancer*
**作者**: Wang, R. et al.
**摘要**: 利用重组LACTB2蛋白处理乳腺癌细胞，发现其通过诱导线粒体分裂抑制肿瘤生长，研究揭示了其重组蛋白在靶向线粒体代谢中的潜在治疗价值。
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**注意**：若需更精准的文献，建议通过PubMed或Google Scholar以“LACTB2 recombinant protein”为关键词检索最新研究，并核实LACTB2与LACTB的命名差异(部分研究可能未明确区分亚型)。
LACTB2 (Lactamase, Beta 2) is a relatively understudied member of the beta-lactamase-like protein family, which shares structural homology with bacterial penicillin-binding proteins and serine proteases. Initially identified through genomic sequencing, LACTB2 is a nuclear-encoded mitochondrial protein implicated in diverse cellular processes, including metabolism, apoptosis, and mitochondrial dynamics. Its name derives from the conserved β-lactamase domain, though it lacks direct enzymatic activity associated with antibiotic resistance. Instead, this domain likely facilitates protein-protein interactions or substrate recognition.
Functionally, LACTB2 is proposed to regulate lipid metabolism by modulating mitochondrial phospholipid composition, potentially influencing membrane fluidity and organelle integrity. Emerging studies link LACTB2 to cancer biology, where it may act as a tumor suppressor by inducing apoptosis and inhibiting proliferation in certain malignancies. Paradoxically, elevated LACTB2 expression has been observed in chemoresistant cancer cells, suggesting context-dependent roles. It also interacts with metabolic enzymes like CHCHD4/MINOS1. hinting at involvement in mitochondrial cristae organization.
Recombinant LACTB2 protein production typically employs E. coli or mammalian expression systems, enabling biochemical characterization and interaction studies. Purified LACTB2 is used to investigate its structural properties, substrate-binding capabilities, and regulatory mechanisms. Researchers utilize it to develop antibodies, study post-translational modifications, and screen for small-molecule modulators in drug discovery. Recent interest focuses on its potential as a biomarker for metabolic disorders and cancer prognosis. However, the protein’s precise molecular targets and signaling pathways remain poorly defined, necessitating further research to clarify its physiological and pathological significance across different tissues and disease states.
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