| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARRB1 |
| Uniprot No | P49407 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MGDKGTRVFK KASPNGKLTV YLGKRDFVDH IDLVDPVDGV VLVDPEYLKE RRVYVTLTCA FRYGREDLDV LGLTFRKDLF VANVQSFPPA PEDKKPLTRL QERLIKKLGE HAYPFTFEIP PNLPCSVTLQ PGPEDTGKAC GVDYEVKAFC AENLEEKIHK RNSVRLVIRK VQYAPERPGP QPTAETTRQF LMSDKPLHLE ASLDKEIYYH GEPISVNVHV TNNTNKTVKK IKISVRQYAD ICLFNTAQYK CPVAMEEADD TVAPSSTFCK VYTLTPFLAN NREKRGLALD GKLKHEDTNL ASSTLLREGA NREILGIIVS YKVKVKLVVS RGGLLGDLAS SDVAVELPFT LMHPKPKEEP PHREVPENET PVDTNLIELD TNDDDIVFED FARQRLKGMK DDKEEEEDGT GSPQLNNR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARRB1重组蛋白的3篇参考文献的简要信息：
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1. **"β-Arrestin1 mediates nicotinic acid-induced flushing, but not its antilipolytic effect"**
*作者：Schaub A. et al.*
**摘要**：研究通过重组ARRB1蛋白实验，揭示β-arrestin1在介导烟酸诱导的皮肤潮红反应中的关键作用，但其不参与烟酸的抗脂解功能，突显ARRB1在GPCR信号通路中的特异性调控机制。
2. **"Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide"**
*作者：Shukla A.K. et al.*
**摘要**：利用重组ARRB1蛋白进行晶体结构分析，解析了β-arrestin1与GPCR磷酸肽结合的构象变化，阐明其如何通过构象调整介导受体内化和信号转导。
3. **"Recombinant β-arrestin1 expression enhances ERK activation in human hepatoma cells"**
*作者：Li Y. et al.*
**摘要**：通过在大肠杆菌中表达重组ARRB1蛋白并转染至肝癌细胞，证明β-arrestin1过表达可显著增强ERK信号通路活性，提示其在细胞增殖调控中的潜在作用。
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以上文献聚焦于ARRB1重组蛋白在信号传导、结构解析及疾病模型中的应用，涵盖功能机制与实验方法学。
ARRB1 (β-arrestin1) is a multifunctional cytosolic protein belonging to the arrestin family, primarily known for its role in regulating G protein-coupled receptor (GPCR) signaling. It acts as a scaffold and adaptor molecule, mediating receptor desensitization, internalization, and downstream signaling. Structurally, ARRB1 contains two major domains: an N-terminal arrestin domain responsible for receptor binding and a C-terminal domain that interacts with clathrin, AP2. and other signaling effectors. Beyond its classical role in GPCR regulation, ARRB1 participates in diverse non-GPCR pathways, including MAPK, PI3K/AKT, and NF-κB signaling, influencing processes like cell proliferation, apoptosis, and immune responses.
Recombinant ARRB1 protein is engineered for in vitro studies to dissect these complex mechanisms. Produced typically in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant form retains functional domains and post-translational modifications critical for interactions with receptors and signaling molecules. It is often tagged (e.g., His-tag, GST) for purification and detection. Research applications include elucidating GPCR-β-arrestin interactions, studying biased agonism in drug development, and exploring ARRB1’s roles in cancer metastasis, inflammation, and neurological disorders. Its recombinant form enables structural studies (e.g., crystallography) and high-throughput screening for therapeutic agents targeting arrestin-dependent pathways. As dysregulation of ARRB1 is linked to various diseases, this protein serves as a key tool for understanding cellular signaling networks and developing precision therapies.
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