| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R1A |
| Uniprot No | Q13522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-171aa |
| 氨基酸序列 | MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTET QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS V |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R1A重组蛋白的参考文献示例(注：部分文献为模拟概括，实际引用请核对原文)：
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1. **"Recombinant expression and functional characterization of PPP1R1A as a protein phosphatase 1 inhibitor"**
*Johnson, R. et al. (2015). Protein Science.*
**摘要**：研究报道了在大肠杆菌系统中成功表达并纯化重组PPP1R1A蛋白，验证其通过特异性抑制PP1磷酸酶活性调控细胞信号通路的功能，为后续机制研究提供工具。
2. **"Structural insights into PPP1R1A regulation by phosphorylation using recombinant protein crystallography"**
*Smith, L. & Wang, Y. (2018). Journal of Molecular Biology.*
**摘要**：通过重组PPP1R1A蛋白的晶体结构解析，揭示了其磷酸化修饰如何改变构象并增强对PP1的结合抑制能力，阐明了动态调控的分子基础。
3. **"PPP1R1A recombinant protein suppresses tumor growth in ovarian cancer models"**
*Chen, H. et al. (2020). Oncogene.*
**摘要**：利用哺乳动物细胞表达的重组PPP1R1A蛋白进行功能实验，发现其过表达通过抑制PP1活性阻断癌细胞增殖通路，提示其作为潜在治疗靶点。
4. **"Development of a high-yield PPP1R1A recombinant protein production system in Pichia pastoris"**
*Martinez, D. et al. (2022). Biotechnology Reports.*
**摘要**：优化毕赤酵母系统高效分泌表达PPP1R1A重组蛋白，解决了真核翻译后修饰需求，为大规模生产用于药物筛选奠定基础。
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**注意**：以上文献信息为示例性质，实际文献可能需要通过PubMed、Web of Science或Google Scholar检索确认。若研究领域较窄，建议结合具体研究方向扩展关键词(如“PPP1R1A + 重组表达/疾病模型/信号通路”)。
PPP1R1A (Protein Phosphatase 1 Regulatory Subunit 1A), also known as inhibitor-1 (I-1), is a key regulatory protein involved in modulating the activity of protein phosphatase 1 (PP1), a major serine/threonine phosphatase in eukaryotic cells. PP1 plays a critical role in diverse cellular processes, including glycogen metabolism, cell cycle progression, and signal transduction. PPP1R1A acts as a potent endogenous inhibitor of PP1 by binding to its catalytic subunit, thereby fine-tuning phosphatase activity in a phosphorylation-dependent manner. This regulation occurs through a conserved "RVxF" motif in PPP1R1A that interacts with PP1. while its inhibitory activity is triggered by phosphorylation at Thr35 by protein kinase A (PKA).
Recombinant PPP1R1A proteins are engineered to study PP1 regulatory mechanisms, often expressed in bacterial or mammalian systems with tags for purification. These tools enable researchers to dissect PP1-dependent signaling pathways, particularly in contexts like neuronal plasticity, cardiac function, and metabolic disorders. Dysregulation of PPP1R1A has been implicated in diseases such as Alzheimer's, cancer, and diabetes, making its recombinant form valuable for drug discovery and mechanistic studies. Recent studies also explore its role in stress responses and epigenetic regulation, highlighting its broader impact on cellular homeostasis. The development of PPP1R1A mutants (e.g., phosphorylation-deficient variants) further aids in understanding structure-function relationships and therapeutic targeting of PP1-mediated pathways.
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