| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST8SIA1 |
| Uniprot No | Q92185-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 49-356aa |
| 氨基酸序列 | YRLPNEKEIV QGVLQQGTAW RRNQTAARAF RKQMEDCCDP AHLFAMTKMN SPMGKSMWYD GEFLYSFTID NSTYSLFPQA TPFQLPLKKC AVVGNGGILK KSGCGRQIDE ANFVMRCNLP PLSSEYTKDV GSKSQLVTAN PSIIRQRFQN LLWSRKTFVD NMKIYNHSYI YMPAFSMKTG TEPSLRVYYT LSDVGANQTV LFANPNFLRS IGKFWKSRGI HAKRLSTGLF LVSAALGLCE EVAIYGFWPF SVNMHEQPIS HHYYDNVLPF SGFHAMPEEF LQLWYLHKIG ALRMQLDPCE DTSLQPTS |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ST8SIA1重组蛋白的模拟参考文献示例(仅供参考，非真实文献)：
1. **文献名称**：*ST8SIA1重组蛋白在肿瘤细胞迁移中的功能研究*
**作者**：Smith A, et al.
**摘要**：本研究通过体外表达ST8SIA1重组蛋白，发现其通过增强细胞表面多聚唾液酸化修饰促进肿瘤细胞迁移，并证实其与整合素信号通路的调控相关。
2. **文献名称**：*ST8SIA1重组蛋白的酶活性表征及结构分析*
**作者**：Wang X, et al.
**摘要**：利用昆虫表达系统纯化ST8SIA1重组蛋白，解析其酶动力学参数及底物特异性，并通过X射线晶体学揭示其催化结构域的关键氨基酸位点。
3. **文献名称**：*ST8SIA1重组蛋白对神经突触可塑性的影响*
**作者**：Tanaka K, et al.
**摘要**：在小鼠神经元模型中，外源性添加ST8SIA1重组蛋白显著促进突触形成，提示其在神经发育和修复中的潜在应用价值。
4. **文献名称**：*ST8SIA1重组蛋白的免疫调节功能研究*
**作者**：Lee JH, et al.
**摘要**：实验表明，ST8SIA1重组蛋白通过调控T细胞表面唾液酸化水平抑制过度免疫反应，为自身免疫疾病治疗提供了新思路。
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注：以上内容为示例性模拟，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
ST8SIA1. also known as GD3 synthase, is a key enzyme in the biosynthesis of gangliosides, a family of sialic acid-containing glycosphingolipids. This transmembrane protein catalyzes the transfer of sialic acid residues to specific glycolipids and glycoproteins, particularly synthesizing GD3 and GT3 gangliosides through α2.8-sialylation reactions. It plays crucial roles in cellular processes including neuronal development, cell adhesion, and signal transduction.
The recombinant ST8SIA1 protein is engineered for research applications to study its enzymatic activity, structural characteristics, and biological functions. Produced typically in mammalian or insect expression systems, it retains the functional domains required for substrate recognition and catalytic activity. Researchers utilize this purified protein to investigate ganglioside metabolism abnormalities linked to neurological disorders, cancer progression, and autoimmune diseases.
Notably, ST8SIA1 overexpression has been associated with tumor malignancy in neuroblastoma, melanoma, and glioblastoma, making its recombinant form valuable for developing therapeutic inhibitors or diagnostic biomarkers. In neuroscience, it helps explore synaptic plasticity mechanisms and neural repair processes. The protein's structure-function relationship is also studied to design modulators of ganglioside-mediated pathways. Current investigations focus on its role in regulating cell migration, apoptosis resistance, and immune evasion in pathological conditions.
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