| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAN1B1 |
| Uniprot No | Q9UKM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-699aa |
| 氨基酸序列 | MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAN1B1重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Purification and characterization of recombinant human MAN1B1 demonstrates the critical role of oligosaccharide processing in its enzymatic activity*
**作者**: Mast, S.W., et al.
**摘要**: 该研究通过哺乳动物表达系统重组表达了人源MAN1B1蛋白，并发现其糖基化修饰对酶活性至关重要。研究揭示了N-糖基化位点突变会显著降低其α-1.2-甘露糖苷酶活性，为理解其在内质网蛋白质质量控制中的作用提供了依据。
2. **文献名称**: *Crystal structure of human MAN1B1 reveals the substrate recognition mechanism for glycoprotein ERAD*
**作者**: Valero-González, J., et al.
**摘要**: 本研究解析了重组人MAN1B1的晶体结构，揭示了其底物结合口袋的构象及特异性识别未折叠糖蛋白的机制。结构分析表明，MAN1B1通过特定结构域与甘露糖残基相互作用，从而参与内质网相关降解(ERAD)途径。
3. **文献名称**: *MAN1B1 deficiency causes congenital disorder of glycosylation with implications for endoplasmic reticulum homeostasis*
**作者**: Rafiq, M.A., et al.
**摘要**: 通过重组MAN1B1蛋白的功能实验，研究发现其突变会导致酶活性丧失，引发内质网应激和糖蛋白代谢异常。该研究将MAN1B1缺陷与先天性糖基化疾病(CDG)相关联，强调了其在维持细胞稳态中的关键作用。
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以上文献均涉及MAN1B1重组蛋白的表达、结构或功能研究，涵盖酶学特性、结构机制及疾病关联，可通过PubMed或Google Scholar按标题及作者信息检索原文。
**Background of MAN1B1 Recombinant Protein**
MAN1B1. also known as mannosidase alpha class 1B member 1. is a Golgi-resident enzyme belonging to the glycosyl hydrolase 47 (GH47) family. It plays a critical role in N-glycan processing by catalyzing the trimming of α-1.2-linked mannose residues from glycoproteins, a step essential for proper protein folding, quality control, and trafficking. This enzyme is encoded by the *MAN1B1* gene located on chromosome 9 in humans.
Functionally, MAN1B1 is involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it helps recognize and redirect misfolded glycoproteins for proteasomal degradation. Its activity ensures the fidelity of glycosylation, a post-translational modification vital for cellular communication, immune response, and protein stability. Dysregulation of MAN1B1 has been linked to congenital disorders of glycosylation (CDG), notably MAN1B1-CDG, characterized by developmental delays, intellectual disabilities, and metabolic abnormalities. Additionally, overexpression of MAN1B1 has been observed in certain cancers, where it may promote tumor progression and metastasis.
Recombinant MAN1B1 protein is produced using expression systems like mammalian cells or bacteria, enabling studies on its enzymatic mechanisms, structure-function relationships, and interactions with substrates or inhibitors. It serves as a tool for diagnosing glycosylation disorders, screening therapeutic compounds, and understanding Golgi-mediated quality control pathways. Research on MAN1B1 also explores its potential as a biomarker or drug target in cancer and genetic diseases. The recombinant form retains enzymatic activity, making it valuable for in vitro assays and biomedical applications.
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