| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KEL |
| Uniprot No | P23276 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-732aa |
| 氨基酸序列 | MEGGDQSEEEPRERSQAGGMGTLWSQESTPEERLPVEGSRPWAVARRVLT AILILGLLLCFSVLLFYNFQNCGPRPCETSVCLDLRDHYLASGNTSVAPC TDFFSFACGRAKETNNSFQELATKNKNRLRRILEVQNSWHPGSGEEKAFQ FYNSCMDTLAIEAAGTGPLRQVIEELGGWRISGKWTSLNFNRTLRLLMSQ YGHFPFFRAYLGPHPASPHTPVIQIDQPEFDVPLKQDQEQKIYAQIFREY LTYLNQLGTLLGGDPSKVQEHSSLSISITSRLFQFLRPLEQRRAQGKLFQ MVTIDQLKEMAPAIDWLSCLQATFTPMSLSPSQSLVVHDVEYLKNMSQLV EEMLLKQRDFLQSHMILGLVVTLSPALDSQFQEARRKLSQKLRELTEQPP MPARPRWMKCVEETGTFFEPTLAALFVREAFGPSTRSAAMKLFTAIRDAL ITRLRNLPWMNEETQNMAQDKVAQLQVEMGASEWALKPELARQEYNDIQL GSSFLQSVLSCVRSLRARIVQSFLQPHPQHRWKVSPWDVNAYYSVSDHVV VFPAGLLQPPFFHPGYPRAVNFGAAGSIMAHELLHIFYQLLLPGGCLACD NHALQEAHLCLKRHYAAFPLPSRTSFNDSLTFLENAADVGGLAIALQAYS KRLLRHHGETVLPSLDLSPQQIFFRSYAQVMCRKPSPQDSHDTHSPPHLR VHGPLSSTPAFARYFRCARGALLNPSSRCQLW |
| 预测分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与KEL重组蛋白相关的3篇文献摘要信息：
1. **文献名称**: "Expression and characterization of recombinant Kell blood group protein"
**作者**: Lee S, et al.
**摘要**: 研究利用哺乳动物表达系统成功表达重组KEL蛋白，验证其抗原性与天然蛋白一致，为血型抗体检测提供标准化工具。
2. **文献名称**: "Structural analysis of the Kell blood group glycoprotein by recombinant expression"
**作者**: Yu LC, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达KEL重组蛋白，解析其跨膜结构域及抗原表位，揭示与溶血性疾病相关的分子构象特征。
3. **文献名称**: "Functional characterization of recombinant KEL protein in endothelial cell adhesion"
**作者**: Claperon A, et al.
**摘要**: 研究证实重组KEL蛋白通过整合素结合通路调控内皮细胞黏附功能，提示其在血管生物学中的潜在作用。
注：以上为模拟文献概括，实际文献需通过PubMed或Web of Science等数据库检索。建议使用关键词 "Kell glycoprotein recombinant" 或 "KEL blood group system recombinant expression" 获取最新研究。
**Background of KEL Recombinant Proteins**
The KEL glycoprotein, encoded by the *KEL* gene on chromosome 11. is a clinically significant blood group antigen system in humans. It belongs to the M13 metalloprotease family and plays a role in erythrocyte membrane integrity and enzymatic processing of surface proteins during red blood cell (RBC) maturation. The KEL system comprises over 35 antigens, with the K (Kell) and k (Cellano) antigens being the most immunogenic. These antigens are critical in transfusion medicine, as mismatches can trigger hemolytic transfusion reactions or hemolytic disease of the fetus/newborn (HDFN).
Traditional methods to study KEL antigens rely on RBCs from rare donors or monoclonal antibodies, which face limitations in scalability and specificity. Recombinant KEL proteins, produced via genetic engineering in host systems (e.g., mammalian cells), overcome these challenges by enabling large-scale, controlled production of antigenic epitopes. These proteins retain the structural and functional features of native KEL glycoproteins, including post-translational modifications essential for antigenicity.
KEL recombinant proteins are pivotal in developing antibody detection assays, diagnostic reagents, and blood-typing tools. They also aid in studying alloimmunization mechanisms and designing therapeutic strategies, such as blocking antibodies in HDFN. Recent advances in CRISPR/Cas9 and expression systems have enhanced the precision and yield of recombinant KEL proteins, supporting their use in personalized transfusion medicine and immunological research. However, challenges remain in mimicking complex RBC membrane environments and ensuring consistent antigen presentation. Overall, KEL recombinant proteins represent a transformative tool for improving transfusion safety and understanding erythrocyte biology.
(Word count: 250)
×
