| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GC |
| Uniprot No | P02774 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MKRVLVLLLAVAFGHALERGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPTELAKLVNKHSDFASNCCSINSPPLYCDSEIDAELKNIL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于GC重组蛋白的示例参考文献(内容为虚构，仅供示例参考)：
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1. **文献名称**：*Optimization of Recombinant GC Protein Expression in Mammalian Cells*
**作者**：Zhang L. et al.
**摘要**：研究通过优化哺乳动物细胞培养条件和载体设计，显著提高了重组GC(鸟苷酸环化酶)蛋白的表达效率，并验证了其生物活性在信号通路中的作用。
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2. **文献名称**：*Therapeutic Potential of Recombinant GC Fusion Proteins in Cardiovascular Diseases*
**作者**：Martinez R., Kim S.
**摘要**：开发了一种重组GC融合蛋白，通过增强cGMP信号通路，在小鼠模型中有效缓解高血压和心肌肥厚，展示了其在心血管疾病治疗中的应用前景。
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3. **文献名称**：*Structural and Functional Characterization of a Novel Recombinant GC-Binding Protein*
**作者**：Watanabe H. et al.
**摘要**：利用X射线晶体学解析了重组GC结合蛋白的三维结构，并发现其通过特异性识别DNA的GC富集区域调控基因表达，为癌症靶向治疗提供新思路。
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注：以上文献为模拟示例，实际引用时需检索真实数据库(如PubMed、Web of Science)获取权威信息。
**Background of GC Recombinant Proteins**
GC recombinant proteins are engineered versions of the group-specific component (GC) protein, a multifunctional glycoprotein encoded by the *GC* gene in humans. Naturally, GC protein, also known as vitamin D-binding protein (DBP), plays a critical role in vitamin D metabolism by binding and transporting vitamin D metabolites in the bloodstream. It also participates in immune modulation, actin scavenging, and inflammatory responses. Structurally, GC protein belongs to the albumin gene family and exhibits genetic polymorphism, with common variants (GC1s, GC1f, GC2) influencing its functional properties.
The development of recombinant GC proteins leverages biotechnology to produce purified, standardized versions of the protein. Recombinant DNA techniques involve inserting the *GC* gene into expression systems (e.g., bacterial, yeast, or mammalian cells) to enable large-scale production. This approach overcomes limitations of natural GC protein isolation, such as low yield, batch variability, and contamination risks.
GC recombinant proteins have gained attention for therapeutic and diagnostic applications. In medicine, they are explored as drug carriers for vitamin D analogs in osteoporosis treatment, anti-inflammatory agents, or immune modulators in autoimmune diseases. Their actin-scavenging properties also make them candidates for mitigating conditions like sepsis or tissue injury. In diagnostics, recombinant GC proteins serve as reliable standards for assays measuring vitamin D status or genetic variations linked to diseases.
Challenges include optimizing post-translational modifications (e.g., glycosylation) to mimic native protein functionality and ensuring stability during production. Ongoing research focuses on engineering GC variants with enhanced binding affinity or tailored functions. Overall, GC recombinant proteins represent a versatile tool bridging biochemistry, clinical research, and therapeutic innovation, with potential to address unmet needs in precision medicine and biopharmaceutical development.
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