| 纯度 | >98%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VNN2 |
| Uniprot No | O95498 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MVTSSFPISV AVFALITLQV GTQDSFIAAV YEHAVILPNK TETPVSQEDA LNLMNENIDI LETAIKQAAE QGARIIVTPE DALYGWKFTR ETVFPYLEDI PDPQVNWIPC QDPHRFGHTP VQARLSCLAK DNSIYVLANL GDKKPCNSRD STCPPNGYFQ YNTNVVYNTE GKLVARYHKY HLYSEPQFNV PEKPELVTFN TAFGRFGIFT CFDIFFYDPG VTLVKDFHVD TILFPTAWMN VLPLLTAIEF HSAWAMGMGV NLLVANTHHV SLNMTGSGIY APNGPKVYHY DMKTELGKLL LSEVDSHPLS SLAYPTAVNW NAYATTIKPF PVQKNTFRGF ISRDGFNFTE LFENAGNLTV CQKELCCHLS YRMLQKEENE VYVLGAFTGL HGRRRREYWQ VCTLLKCKTT NLTTCGRPVE TASTRFEMFS LSGTFGTEYV FPEVLLTEIH LSPGKFEVLK DGRLVNKNGS SGPILTVSLF GRWYTKDSLY SS |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VNN2重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库查询)：
---
1. **文献名称**: "Recombinant VNN2 Protein Attenuates Oxidative Stress in Macrophages via Pantetheinase Activity"
**作者**: Smith A, et al. (2018)
**摘要**: 研究通过大肠杆菌表达系统制备重组VNN2蛋白，发现其通过水解泛酰巯基乙胺生成半胱胺，显著降低巨噬细胞内的活性氧(ROS)水平，提示其在调控氧化应激中的潜在作用。
2. **文献名称**: "Structural Characterization of Human Vanin-2 and Its Interaction with TLR4 in Inflammatory Pathways"
**作者**: Johnson R, et al. (2020)
**摘要**: 利用X射线晶体学解析了重组VNN2的蛋白结构，揭示其与Toll样受体4(TLR4)的相互作用界面，为开发靶向VNN2-TLR4通路的抗炎药物提供了结构基础。
3. **文献名称**: "VNN2 as a Biomarker in Colorectal Cancer: Expression Analysis Using Recombinant Protein-based ELISA"
**作者**: Chen L, et al. (2019)
**摘要**: 通过哺乳动物细胞表达系统生产高纯度重组VNN2.开发特异性ELISA检测方法，发现结直肠癌患者血清VNN2水平显著升高，提示其作为诊断标记物的潜力。
4. **文献名称**: "Recombinant VNN2-Fc Fusion Protein Ameliorates Metabolic Dysfunction in Obese Mouse Models"
**作者**: Lee H, et al. (2021)
**摘要**: 构建VNN2与IgG Fc段的融合蛋白，证明其通过调节脂代谢相关基因(如PPARγ)改善肥胖小鼠的胰岛素抵抗和肝脏脂肪变性，为代谢性疾病治疗提供新策略。
---
建议通过PubMed、Web of Science或Google Scholar以关键词“VNN2 recombinant protein”“Vanin-2 expression”等检索最新文献，获取权威数据。
**Background of VNN2 Recombinant Protein**
VNN2 (Vanin-2), also known as pantetheinase, is a glycosylphosphatidylinositol (GPI)-anchored protein encoded by the *VNN2* gene. It belongs to the vanin family of ectoenzymes, which includes VNN1 and VNN3 in humans. VNN2 is primarily expressed on the surface of immune cells, epithelial cells, and endothelial cells, playing a critical role in inflammation, oxidative stress responses, and cellular migration. Its enzymatic activity involves the hydrolysis of pantetheine to produce cysteamine and pantothenic acid (vitamin B5), thereby regulating intracellular glutathione metabolism and redox homeostasis.
Biologically, VNN2 has been implicated in modulating immune responses. It influences leukocyte trafficking by interacting with integrins and chemokines, facilitating immune cell recruitment to sites of inflammation or injury. Studies highlight its dual role in both pro-inflammatory and anti-inflammatory processes, depending on the cellular context. For instance, VNN2 overexpression is associated with chronic inflammatory diseases (e.g., inflammatory bowel disease, atherosclerosis) and certain cancers, where it may promote tumor progression or metastasis. Conversely, its anti-oxidative functions contribute to tissue repair and protection against oxidative damage.
The recombinant VNN2 protein is engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified, functional protein for research and therapeutic applications. It serves as a tool to study VNN2's signaling pathways, ligand interactions, and roles in disease pathogenesis. Additionally, recombinant VNN2 is explored for potential clinical use, such as modulating immune responses in autoimmune disorders or enhancing antioxidant defenses in metabolic diseases.
Overall, VNN2 recombinant protein represents a valuable resource for dissecting the complex biological functions of VNN2 and developing targeted therapies for inflammation-related conditions.
×
