| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DLG4 |
| Uniprot No | P78352-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767aa |
| 氨基酸序列 | MSQRPRAPRSALWLLAPPLLRWAPPLLTVLHSDLFQALLDILDYYEASLS ESQKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGT EGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQ DGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEK VMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQ IGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAP PDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDI PREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGD QILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDL REQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFG DVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDW GSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPD KFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSH LYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLEN VLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRV IEDLSGPYIWVPARERL |
| 预测分子量 | 112 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DLG4重组蛋白的参考文献示例(注：以下为虚构示例，仅展示格式和内容结构)：
1. **标题**：*Recombinant Expression and Functional Analysis of DLG4/PSD-95 in Synaptic Signaling*
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌系统中成功表达并纯化DLG4重组蛋白，验证其与NMDA受体的相互作用，揭示其在突触信号传导中的支架作用。
2. **标题**：*Structural Insights into DLG4 Domains via Recombinant Protein Crystallography*
**作者**：Lee B, et al.
**摘要**：利用昆虫细胞表达系统获得DLG4的PDZ结构域重组蛋白，通过X射线晶体学解析其三维结构，阐明其与配体结合的分子机制。
3. **标题**：*High-Yield Purification of DLG4 for Neurodegenerative Drug Screening*
**作者**：Zhang C, et al.
**摘要**：开发了一种高效的真核表达体系(HEK293细胞)生产DLG4重组蛋白，应用于高通量药物筛选，以靶向突触功能障碍相关疾病。
4. **标题**：*DLG4 Recombinant Protein Modulates Synaptic Plasticity In Vitro*
**作者**：Garcia D, et al.
**摘要**：通过体外重建实验，证明重组DLG4蛋白对突触可塑性的调控作用，为神经退行性疾病研究提供实验模型。
(如需真实文献，建议通过PubMed或Google Scholar以“DLG4 recombinant protein”等关键词检索。)
DLG4 (Discs Large Homolog 4), also known as PSD-95 (Postsynaptic Density Protein 95) or SAP90 (Synapse-Associated Protein 90), is a critical scaffolding protein enriched in the postsynaptic density (PSD) of excitatory neurons. It belongs to the membrane-associated guanylate kinase (MAGUK) family, characterized by three N-terminal PDZ domains, a SH3 domain, and a guanylate kinase (GK)-like domain. These structural features enable DLG4 to orchestrate protein-protein interactions, anchoring receptors (e.g., NMDA and AMPA receptors), ion channels, and signaling molecules at synaptic sites. This organization is vital for synaptic plasticity, neurotransmission, and maintaining neuronal circuit stability.
DLG4 dysfunction has been implicated in neuropsychiatric and neurodegenerative disorders, including autism spectrum disorders, schizophrenia, and Alzheimer’s disease. Its role in regulating synaptic strength and receptor trafficking makes it a focal point for studying mechanisms underlying cognitive processes and disease pathology.
Recombinant DLG4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional protein for research. This recombinant form retains key domains for binding partners, enabling *in vitro* studies of synaptic protein networks, drug screening, and structural analyses. Researchers utilize it to dissect DLG4’s interactions with glutamate receptors, neuroligins, or pathogenic proteins like amyloid-beta. Its applications extend to developing therapeutic strategies targeting synaptic dysfunction. However, challenges remain in preserving post-translational modifications and native conformation during recombinant production. Advances in expression systems and purification techniques continue to enhance the utility of DLG4 recombinant protein in both basic neuroscience and translational research.
×
