| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBB |
| Uniprot No | P0CG47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-76aa |
| 氨基酸序列 | MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL EDGRTLSDYNIQKESTLHLVLRLRGG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBB重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Production and Characterization of Recombinant Human Ubiquitin B (UBB) in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：该研究报道了通过大肠杆菌表达系统高效表达重组人源UBB蛋白，优化了纯化条件，并通过质谱验证了其正确性，为泛素-蛋白酶体功能研究提供了工具。
2. **文献名称**：*UBB+1 Recombinant Protein Induces Tau Aggregation in Neurodegenerative Models*
**作者**：van Leeuwen FW, et al.
**摘要**：研究利用重组UBB+1突变体(阿尔茨海默病相关变异)在神经元细胞模型中验证其异常积累，证明其促进tau蛋白聚集并导致细胞毒性。
3. **文献名称**：*Functional Analysis of Ubiquitin B Chains via Recombinant Expression in Yeast*
**作者**：Johnson ES, et al.
**摘要**：通过酵母系统表达重组UBB蛋白，揭示了泛素链长度对蛋白酶体降解底物的调控机制，阐明了UBB在多聚泛素化中的特异性功能。
(注：上述文献为示例，实际文献需通过PubMed/Google Scholar检索确认。)
Ubiquitin B (UBB) is a member of the ubiquitin family, a highly conserved small protein (76 amino acids) critical for protein homeostasis in eukaryotic cells. As a key component of the ubiquitin-proteasome system (UPS), ubiquitin tags target proteins for degradation via covalent attachment, regulating processes like cell cycle progression, DNA repair, and stress responses. UBB is one of four ubiquitin genes in humans (UBA52. UBB, UBC, and UBB pseudogene 1), with UBB encoding a polyubiquitin precursor protein containing tandem repeats of ubiquitin monomers.
Recombinant UBB protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic (e.g., yeast, mammalian cells) systems. This allows large-scale production of ubiquitin for research and therapeutic applications. Recombinant UBB retains its native structure and biochemical functions, including the ability to conjugate to substrates via E1-E2-E3 enzymatic cascades. Its stability, solubility, and conserved nature make it a valuable tool for studying UPS mechanisms, protein degradation pathways, and ubiquitin-related post-translational modifications.
In neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), aberrant UBB aggregates are linked to UPS dysfunction. Recombinant UBB is used to model these pathologies, screen UPS-targeting drugs, and explore therapies to restore proteostasis. Additionally, it serves as a standard in ubiquitination assays, structural studies (NMR, X-ray crystallography), and proteomics research. Advances in recombinant technology, such as site-specific mutations or isotope labeling, further enhance its utility in dissecting ubiquitin's diverse roles in health and disease.
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