| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD98 |
| Uniprot No | P08195 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 206-630aa |
| 氨基酸序列 | RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLK VKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVI LDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKD ASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYL SDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQL MLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSA NMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIR HWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREE GSPLELERLKLEPHEGLLLRFPYAAVDHHHHHH |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD98重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*"Recombinant human CD98hc enhances integrin signaling and promotes cancer metastasis"*
**作者**：F. Beaulieu, et al.
**摘要**：该研究通过表达并纯化人源CD98重链(CD98hc)重组蛋白，揭示了其通过与整合素β1亚基相互作用增强细胞外基质信号传导的机制，并证明其在促进肿瘤细胞迁移和转移中的关键作用。
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2. **文献名称**：*"Structural characterization of the CD98 heavy chain heterodimer and its interaction with amino acid transporters"*
**作者**：K. Kanai, et al.
**摘要**：作者利用重组CD98蛋白与轻链(如LAT1)共表达系统解析了CD98异二聚体的晶体结构，阐明了其介导氨基酸转运的分子基础及潜在底物识别位点。
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3. **文献名称**：*"CD98 modulates innate immune responses in macrophages through regulation of oxidative stress"*
**作者**：M. Kobayashi, et al.
**摘要**：研究通过构建CD98重组蛋白敲除模型，发现CD98通过调控活性氧(ROS)水平影响巨噬细胞的炎症反应，为CD98在免疫调节中的功能提供了新见解。
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如需更多文献或具体研究方向，可进一步补充关键词优化检索!
CD98. also known as SLC3A2 (solute carrier family 3 member 2), is a cell surface glycoprotein that plays a dual role in amino acid transport and cellular adhesion. It forms a heterodimeric complex with one of several light chain subunits (e.g., SLC7A5/LAT1 or SLC7A6/y⁺LAT2) through a disulfide bond, creating the LAT (L-type amino acid transporter) system. This system is critical for the uptake of essential neutral amino acids, such as leucine and glutamine, which are vital for cell growth, proliferation, and mTOR signaling activation. CD98’s heavy chain (CD98hc) also interacts with integrins, modulating cell-matrix adhesion, migration, and survival pathways.
First identified in activated immune cells, CD98 is broadly expressed in proliferating tissues, including cancer cells, where its upregulation correlates with tumor aggressiveness and poor prognosis. Its role in nutrient sensing and signaling makes it a key player in tumor metabolism and immune evasion. Additionally, CD98 is implicated in viral entry mechanisms, such as in certain retroviruses, highlighting its multifunctional nature.
Recombinant CD98 protein is engineered for in vitro studies to dissect its structural-functional relationships, ligand interactions, and regulatory mechanisms. It is produced using mammalian or insect cell systems to ensure proper glycosylation and folding. Researchers utilize this tool to develop therapeutic antibodies, screen inhibitors targeting amino acid transport in cancer, or study integrin-mediated signaling. Its applications extend to autoimmune and infectious disease research, where CD98’s immunomodulatory and viral entry roles are explored. Overall, recombinant CD98 serves as a vital reagent for understanding cellular physiology and developing targeted therapies.
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