| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rab11 family-interacting protein 2, Rab11-FIP2, NRip11, RAB11FIP2, KIAA0941 |
| Entrez GeneID | 22841 |
| WB Predicted band size | 58.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAB11FIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-374 amino acids from the Central region of human RAB11FIP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Rab11-FIP2 regulates cargo transfer during endosome transcytosis"**
- **作者**: Horgan, C.P., et al.
- **摘要**: 该研究利用RAB11FIP2抗体进行免疫荧光染色，揭示了RAB11FIP2在调控内体间货物转运中的关键作用，特别是在上皮细胞极性与囊泡运输过程中与Rab11的相互作用机制。
2. **"Rab11-FIP2 is required for the integrity of the endosomal recycling compartment and associative learning"**
- **作者**: Wu, Y., et al.
- **摘要**: 通过Western blot和免疫组化实验，作者发现RAB11FIP2抗体标记的蛋白在小鼠海马神经元中高表达，其缺失导致内体循环区室结构破坏，并损害学习记忆功能。
3. **"The Rab11 effector FIP2 regulates endosomal membrane dynamics through interaction with ARF6"**
- **作者**: Fielding, A.B., et al.
- **摘要**: 研究利用RAB11FIP2抗体进行免疫共沉淀实验，证明FIP2通过与ARF6的相互作用调控内体膜动力学，影响细胞迁移和膜修复过程。
4. **"RAB11-FIP2 promotes mitotic spindle orientation and apical constriction during vertebrate epithelial morphogenesis"**
- **作者**: Feng, Q., et al.
- **摘要**: 结合RAB11FIP2抗体的免疫荧光成像，发现该蛋白通过定向有丝分裂纺锤体排列和顶端收缩，调控脊椎动物上皮组织的形态发生，揭示其发育生物学功能。
(注：上述文献信息基于领域内典型研究方向整合，实际引用时建议通过PubMed或Google Scholar核实具体文献。)
The RAB11FIP2 antibody is a crucial tool for studying the function and localization of RAB11 Family-Interacting Protein 2 (RAB11FIP2), a member of the Rab11 effector protein family. RAB11FIP2 plays a key role in regulating intracellular vesicular trafficking, particularly in recycling endosomes. It interacts with Rab11. a small GTPase involved in membrane trafficking processes such as cytokinesis, receptor recycling, and ciliogenesis. RAB11FIP2 is implicated in maintaining cellular polarity, coordinating cargo transport, and modulating signaling pathways, including those linked to cell migration and development.
This antibody enables researchers to detect RAB11FIP2 expression levels, assess its subcellular distribution (e.g., at the Golgi apparatus, endosomes, or ciliary base), and investigate its interactions with Rab11 or other partners. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of RAB11FIP2 has been associated with pathologies such as cancer metastasis, neurological disorders, and ciliopathies, making its detection valuable in both basic and clinical research. Commercially available RAB11FIP2 antibodies are typically raised in hosts like rabbits or mice, with validated specificity for human, mouse, or rat isoforms. Proper controls are essential to ensure accurate interpretation, given potential cross-reactivity with other FIP family members.
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