| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polyprenol reductase, 3-oxo-5-alpha-steroid 4-dehydrogenase 3, Steroid 5-alpha-reductase 2-like, Steroid 5-alpha-reductase 3, S5AR 3, SR type 3, SRD5A3, SRD5A2L |
| Entrez GeneID | 79644 |
| WB Predicted band size | 36.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This SRD5A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human SRD5A3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及SRD5A3抗体的研究文献摘要示例(注：文献信息为虚拟模拟，仅供参考)：
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1. **文献名称**: *SRD5A3 mutations cause congenital disorder of glycosylation through impaired dolichol metabolism*
**作者**: Cantagrel V. et al.
**摘要**: 本研究通过基因测序发现SRD5A3突变导致糖基化障碍(CDG)，利用特异性SRD5A3抗体进行Western blot和免疫荧光实验，证实突变降低了SRD5A3蛋白表达，破坏内质网中多萜醇代谢通路。
2. **文献名称**: *Functional characterization of SRD5A3 deficiency in a zebrafish model*
**作者**: Kahrizi K. et al.
**摘要**: 通过斑马鱼模型研究SRD5A3功能缺失的影响，使用抗SRD5A3抗体进行组织定位分析，发现其广泛表达于神经和肝脏组织，突变导致GPI锚定蛋白合成异常及胚胎发育缺陷。
3. **文献名称**: *Antibody-based detection of SRD5A3 in human fibroblasts*
**作者**: Morava E. et al.
**摘要**: 本文开发了一种高特异性SRD5A3单克隆抗体，用于患者成纤维细胞分析，发现SRD5A3蛋白表达缺失与α-二葡糖基转移酶活性降低直接相关，为CDG诊断提供生物标志物。
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如需获取真实文献，建议通过PubMed或Google Scholar检索关键词“SRD5A3 antibody”或结合具体研究主题筛选。
The SRD5A3 (steroid 5α-reductase type 3) antibody is a tool used to study the enzyme encoded by the SRD5A3 gene, which plays a critical role in steroid metabolism and protein glycosylation. SRD5A3 catalyzes the conversion of polyprenols to dolichols, essential lipid carriers for oligosaccharide synthesis in N-glycosylation pathways. This enzyme is vital for post-translational modification of proteins, impacting cellular functions like cell signaling, adhesion, and immune responses. Mutations in SRD5A3 are linked to congenital disorder of glycosylation type 1Q (CDG-IQ), characterized by developmental delays, neurological deficits, and ocular abnormalities.
The SRD5A3 antibody detects the presence and distribution of the SRD5A3 protein in tissues or cells, aiding research into its physiological roles and disease mechanisms. It is utilized in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression levels, subcellular localization, and potential dysregulation in pathological conditions. Studies using this antibody contribute to understanding SRD5A3's involvement in glycosylation defects, metabolic disorders, and potential therapeutic targets. Its specificity and reliability make it valuable for both basic research and clinical investigations into SRD5A3-related diseases.
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