| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRISP3 |
| Uniprot No | P54108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-245aa |
| 氨基酸序列 | NEDKDPAFTALLTTQTQVQREIVNKHNELRRAVSPPARNMLKMEWNKEAA ANAQKWANQCNYRHSNPKDRMTSLKCGENLYMSSASSSWSQAIQSWFDEY NDFDFGVGPKTPNAVVGHYTQVVWYSSYLVGCGNAYCPNQKVLKYYYVCQ YCPAGNWANRLYVPYEQGAPCASCPDNCDDGLCTNGCKYEDLYSNCKSLK LTLTCKHQLVRDSCKASCNCSNSIYVDHHHHHH |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRISP3重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*CRISP3 promotes prostate cancer metastasis via autocrine signaling*
**作者**：A. Udby et al.
**摘要**：研究通过重组CRISP3蛋白实验，发现其在前列腺癌细胞中激活MAPK信号通路，促进细胞迁移和侵袭，提示其在肿瘤转移中的潜在作用。
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2. **文献名称**：*Recombinant CRISP3 modulates sperm capacitation and fertilization in vitro*
**作者**：M. Da Ros et al.
**摘要**：利用重组CRISP3蛋白模拟生殖环境，发现其通过调控离子通道影响精子获能过程，为CRISP3在雄性生殖系统中的功能提供机制解释。
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3. **文献名称**：*CRISP3 as a biomarker in pancreatic cancer: Expression and functional validation*
**作者**：S. Li et al.
**摘要**：通过体外表达重组CRISP3.证实其在胰腺癌细胞中高表达，并增强肿瘤细胞对化疗药物的抵抗性，提示其作为治疗靶点的潜力。
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如需更具体的研究细节或扩展领域文献，可进一步补充。
**CRISP3 Recombinant Protein: Background Overview**
CRISP3 (Cysteine-Rich Secretory Protein 3) belongs to the CRISP family, a group of proteins characterized by conserved cysteine-rich domains. These proteins are widely distributed across vertebrates and are implicated in diverse physiological processes, including reproduction, innate immunity, and cancer progression. CRISP3. specifically, is encoded by the *CRISP3* gene and is expressed in secretory tissues such as salivary glands, pancreas, and prostate, as well as in immune cells.
Structurally, CRISP3 contains an N-terminal pathogenesis-related (PR-1) domain and a C-terminal cysteine-rich domain (CAP domain), which likely mediate its interactions with ion channels, proteases, or extracellular matrix components. Its biological roles remain under investigation, but studies suggest involvement in sperm maturation, fertilization, and modulation of immune responses. For example, CRISP3 binds to substrates like kallikrein-related peptidases, potentially regulating proteolytic cascades in inflammation or tissue remodeling.
Recombinant CRISP3 protein is produced using biotechnological platforms (e.g., *E. coli*, mammalian cells) to enable functional studies. By cloning the *CRISP3* gene into expression vectors, researchers generate purified, bioactive protein for investigating its molecular mechanisms, structural properties, and interactions. This recombinant tool has proven vital in dissecting CRISP3's dual roles in health and disease. Notably, elevated CRISP3 levels are linked to cancers (e.g., prostate, pancreatic), where it may promote invasion or resistance to therapy, making it a potential biomarker or therapeutic target.
Current research focuses on clarifying CRISP3's signaling pathways and therapeutic potential, particularly in oncology and reproductive medicine. Its recombinant form also aids in developing diagnostic assays or inhibitors. However, challenges remain in fully elucidating its context-dependent functions, necessitating further *in vivo* and clinical studies. Overall, CRISP3 recombinant protein serves as a critical resource for advancing both basic and translational research.
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