| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Helicase POLQ-like, Mus308-like helicase, POLQ-like helicase, HELQ, HEL308 |
| Entrez GeneID | 113510 |
| WB Predicted band size | 124.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This HEL308 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the Central region of human HEL308. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HEL308(HELQ)抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51*
**作者**: Tkáč J. et al.
**摘要**: 该研究揭示了HELQ(HEL308)在DNA双链断裂修复中的双重功能，通过RPA和RAD51调控。作者利用特异性抗体证实HELQ在复制应激条件下招募至损伤位点，促进同源重组修复，并证明其解旋酶活性对维持基因组稳定性至关重要。
2. **文献名称**: *HELQ suppresses ovarian cancer progression by enhancing DNA damage response*
**作者**: Zhang L. et al.
**摘要**: 研究通过免疫组化(使用HEL308抗体)分析卵巢癌组织，发现HELQ低表达与患者预后不良相关。机制研究表明，HELQ通过增强ATM/ATR介导的DNA损伤应答抑制肿瘤转移，提示其作为潜在治疗靶点。
3. **文献名称**: *HEL308 helicase interacts with Fanconi anemia core complex to resolve replication stress*
**作者**: Higgs MR. et al.
**摘要**: 该文献报道HEL308与范可尼贫血核心蛋白复合物(FA complex)互作，通过免疫共沉淀(使用HEL308抗体)证实其在复制叉重启中的作用。研究表明，HEL308缺失导致复制叉停滞加剧，加剧染色体不稳定性。
4. **文献名称**: *The helicase HELQ promotes cellular tolerance to cisplatin treatment in human lung cancer*
**作者**: Wang Y. et al.
**摘要**: 研究利用HEL308抗体敲低实验发现，HELQ通过调控跨损伤合成(TLS)通路增强肺癌细胞对顺铂的耐受性。HELQ缺失可提高化疗敏感性，为联合治疗策略提供新思路。
**注**：以上文献名称与摘要内容为示例性质，实际文献需通过数据库(如PubMed)检索确认。HEL308相关研究多集中于其在DNA修复及肿瘤中的功能，抗体常用于定位、表达水平检测及互作研究。
HEL308. also known as HELQ or Helicase POLQ-like, is a DNA helicase involved in genome maintenance and repair. Initially identified in *C. elegans* and later characterized in humans, it belongs to the Ski2-like helicase family and plays roles in homologous recombination repair (HRR) and replication fork stabilization. HEL308 unwinds DNA secondary structures and displaced strands during replication stress, facilitating error-free DNA damage repair.
Studies suggest HEL308 interacts with key HRR proteins like RAD51. though its precise mechanism remains under investigation. It may suppress excessive recombination by disrupting RAD51 filaments, balancing repair accuracy and efficiency. HEL308 deficiency correlates with genomic instability, increasing cancer susceptibility.
In cancer research, HEL308 is explored as a synthetic lethal target, particularly in HRR-deficient tumors (e.g., BRCA-mutated cancers). Inhibiting HEL308 in such contexts may enhance PARP inhibitor efficacy or chemotherapeutic responses. Additionally, HEL308’s role in meiosis—evidenced by infertility in HELQ-deficient mice—highlights its importance in germ cell development.
Antibodies against HEL308 are vital tools for studying its expression, localization, and interactions. They aid in diagnosing DNA repair defects and evaluating therapeutic biomarkers. Current research focuses on HEL308’s dual roles in tumor suppression and therapy resistance, positioning it as a potential target for precision oncology.
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