| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Tie1 |
| Uniprot No | P35590 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-760aa |
| 氨基酸序列 | AVDLTLLANL RLTDPQRFFL TCVSGEAGAG RGSDAWGPPL LLEKDDRIVR TPPGPPLRLA RNGSHQVTLR GFSKPSDLVG VFSCVGGAGA RRTRVIYVHN SPGAHLLPDK VTHTVNKGDT AVLSARVHKE KQTDVIWKSN GSYFYTLDWH EAQDGRFLLQ LPNVQPPSSG IYSATYLEAS PLGSAFFRLI VRGCGAGRWG PGCTKECPGC LHGGVCHDHD GECVCPPGFT GTRCEQACRE GRFGQSCQEQ CPGISGCRGL TFCLPDPYGC SCGSGWRGSQ CQEACAPGHF GADCRLQCQC QNGGTCDRFS GCVCPSGWHG VHCEKSDRIP QILNMASELE FNLETMPRIN CAAAGNPFPV RGSIELRKPD GTVLLSTKAI VEPEKTTAEF EVPRLVLADS GFWECRVSTS GGQDSRRFKV NVKVPPVPLA APRLLTKQSR QLVVSPLVSF SGDGPISTVR LHYRPQDSTM DWSTIVVDPS ENVTLMNLRP KTGYSVRVQL SRPGEGGEGA WGPPTLMTTD CPEPLLQPWL EGWHVEGTDR LRVSWSLPLV PGPLVGDGFL LRLWDGTRGQ ERRENVSSPQ ARTALLTGLT PGTHYQLDVQ LYHCTLLGPA SPPAHVLLPP SGPPAPRHLH AQALSDSEIQ LTWKHPEALP GPISKYVVEV QVAGGAGDPL WIDVDRPEET STIIRGLNAS TRYLFRMRAS IQGLGDWSNT VEESTLGNGL QAEGPVQESR AAEEGLDQQV DHHHHHH |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Tie1重组蛋白的3篇参考文献及简要摘要：
1. **"Structural basis of Tie1 ectodomain recognition by angiopoietins"**
- **作者**: Kim M, et al.
- **摘要**: 通过X射线晶体学解析Tie1重组胞外域与血管生成素配体的结合模式，揭示了Tie1特异性识别Ang2的结构基础，为靶向血管生成的治疗设计提供依据。
2. **"Tie1 regulates endothelial cell function via modulating Tie2 signaling"**
- **作者**: Savant S, et al.
- **摘要**: 利用Tie1重组蛋白探究其与Tie2受体的相互作用，发现Tie1通过抑制Tie2过度激活维持血管稳定性，为血管疾病机制研究提供新视角。
3. **"Recombinant Tie1-Fc fusion protein attenuates tumor angiogenesis"**
- **作者**: Chen H, et al.
- **摘要**: 构建Tie1胞外域-Fc融合蛋白，证明其通过竞争性结合血管生成素抑制肿瘤血管异常增生，在动物模型中显著降低肿瘤生长速率。
*注：若需获取全文，建议通过PubMed或Sci-Hub输入DOI或标题查询。*
Tie1 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 1) is a transmembrane receptor tyrosine kinase (RTK) predominantly expressed in endothelial cells, playing a critical role in vascular development and homeostasis. Discovered in 1992. Tie1 is part of the Tie receptor family, alongside Tie2 (TEK). While Tie2 is well-characterized with known ligands (angiopoietins), Tie1's ligand remains elusive, making its signaling mechanisms less understood. Structurally, Tie1 features an extracellular region containing immunoglobulin-like domains, EGF-like repeats, and fibronectin type III motifs, coupled with an intracellular tyrosine kinase domain.
Tie1 is essential for embryonic angiogenesis, as Tie1 knockout mice exhibit embryonic lethality due to vascular defects, including hemorrhage and edema. It regulates endothelial cell survival, vascular integrity, and quiescence, often interacting with Tie2 or modulating angiopoietin-Tie2 signaling. In adults, Tie1 is implicated in pathological angiogenesis, inflammation, and vascular remodeling, with dysregulation linked to cancer, atherosclerosis, and pulmonary hypertension.
Recombinant Tie1 proteins, typically produced in mammalian or insect cell systems, retain functional extracellular domains for ligand-binding studies or structural analysis. These tools enable research into Tie1's role in signaling crosstalk, endothelial barrier function, and mechanotransduction. Additionally, recombinant Tie1 is used to screen therapeutic agents targeting angiogenesis or vascular leakage. Despite progress, Tie1's orphan receptor status and context-dependent signaling (pro-angiogenic vs. stabilizing) remain key challenges. Unraveling Tie1's biology through recombinant protein-based approaches holds promise for therapies addressing vascular disorders and cancer.
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