| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-27B, C25KG, RAB27B |
| Entrez GeneID | 5874 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAB27B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human RAB27B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB27B抗体的模拟参考文献示例(仅供参考，非真实文献)：
1. **"RAB27B regulates exosome secretion in breast cancer metastasis"**
*作者：Zhang Y, et al.*
**摘要**：本研究利用RAB27B特异性抗体，通过Western blot和免疫荧光技术，揭示了RAB27B在乳腺癌细胞外泌体分泌中的关键作用，其表达水平与肿瘤转移能力呈正相关。
2. **"RAB27B-mediated vesicle trafficking promotes melanoma cell invasion"**
*作者：Hendrix A, et al.*
**摘要**：通过siRNA敲低和RAB27B抗体标记实验，证明RAB27B通过调控囊泡运输介导黑色素瘤细胞基质降解蛋白的释放，从而增强肿瘤侵袭性。
3. **"Role of RAB27B in platelet dense granule secretion"**
*作者：Tolmachova T, et al.*
**摘要**：利用RAB27B抗体进行亚细胞定位分析，发现RAB27B缺失导致血小板致密颗粒分泌异常，揭示了其在凝血功能中的新机制。
4. **"RAB27B expression correlates with poor prognosis in gastric cancer"**
*作者：Wang L, et al.*
**摘要**：基于免疫组化(使用RAB27B抗体)和大样本临床数据分析，证实RAB27B在胃癌组织中高表达，且与患者生存期缩短显著相关。
*注：以上为示例性内容，实际文献需通过数据库(如PubMed)检索确认。*
The RAB27B antibody is a research tool designed to detect and study RAB27B, a small GTPase belonging to the RAB family of proteins involved in intracellular vesicle trafficking and membrane dynamics. RAB27B plays a critical role in regulating secretory pathways, including melanosome transport, lysosome-related organelle positioning, and the release of secretory granules in specialized cells. It is closely related to RAB27A, sharing structural homology but exhibiting distinct tissue-specific expression patterns and functional roles. Dysregulation of RAB27B has been implicated in cancers (e.g., breast, prostate) and neurological disorders, making it a target for studying disease mechanisms.
RAB27B antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to localize the protein, assess expression levels, or investigate interactions with effector molecules (e.g., Slp4. MyRIP). These antibodies are typically raised against specific epitopes, such as the C-terminal or N-terminal regions, to ensure specificity. Validation includes testing in knockout cell lines to confirm minimal cross-reactivity with RAB27A or other RAB proteins. Researchers rely on these antibodies to explore RAB27B’s role in exosome secretion, cell-cell communication, and autophagy, offering insights into therapeutic strategies targeting vesicular trafficking pathways. Commercial antibodies are available from suppliers like Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, with host species (rabbit, mouse) and clonality (monoclonal/polyclonal) varying by product.
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