| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable ATP-dependent RNA helicase DDX49, DEAD box protein 49, DDX49 |
| Entrez GeneID | 54555 |
| WB Predicted band size | 54.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DDX49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-413 amino acids from the C-terminal region of human DDX49. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDX49抗体的示例性参考文献(注：以下内容为示例，实际文献需通过学术数据库检索验证)：
1. **文献名称**：*DDX49: A DEAD-box RNA Helicase Involved in Antiviral Immunity*
**作者**：Smith A, et al.
**摘要**：本研究鉴定了DDX49蛋白在哺乳动物细胞抗病毒天然免疫应答中的作用，开发了针对DDX49的多克隆抗体，并通过免疫共沉淀和Western blot验证其特异性，发现DDX49通过调控RIG-I信号通路参与RNA病毒防御。
2. **文献名称**：*Characterization of a Monoclonal Antibody against Human DDX49 for Cancer Biomarker Studies*
**作者**：Li X, et al.
**摘要**：报道了一种高特异性抗人DDX49单克隆抗体的制备及验证，该抗体成功应用于免疫组化检测多种肿瘤组织样本，提示DDX49在结直肠癌中异常高表达，可能与RNA代谢失调相关。
3. **文献名称**：*DDX49 Antibody Application in Subcellular Localization Analysis*
**作者**：Garcia R, et al.
**摘要**：利用DDX49特异性抗体进行亚细胞定位研究，发现该蛋白主要定位于细胞核仁，并通过siRNA敲低实验证实其参与核糖体RNA前体加工过程。
4. **文献名称**：*Cross-species Reactivity of Commercial DDX49 Antibodies: A Comparative Study*
**作者**：Wang Y, et al.
**摘要**：系统评估了市售DDX49抗体在小鼠、斑马鱼等模式生物中的交叉反应性，发现部分抗体存在非特异性结合，为相关研究提供了抗体选择的技术参考。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以“DDX49 antibody”、“DEAD-box protein 49”为关键词检索。若研究较少，可扩展至“DEAD-box protein + RNA helicase + antibody”等方向。
**Background of DDX49 Antibody**
DDX49 is a member of the DEAD-box family of RNA helicases, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. These ATP-dependent enzymes play critical roles in RNA metabolism, including ribosome biogenesis, RNA splicing, transport, and translation. DDX49 is implicated in facilitating ribosomal RNA (rRNA) processing and nucleocytoplasmic transport of ribosomal subunits, linking it to cellular proliferation and stress responses.
Antibodies targeting DDX49 are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in the exploration of DDX49’s role in ribosome assembly and RNA homeostasis. Research suggests DDX49 may contribute to pathological conditions, including viral infections (e.g., HIV-1) and cancers, where dysregulated RNA processing drives disease progression.
DDX49 antibodies are often validated for specificity across species (e.g., human, mouse) and applied in both basic and translational research. Recent studies highlight its interaction with viral RNAs or involvement in stress granule formation, underscoring its potential as a therapeutic target. However, functional insights remain incomplete, necessitating further investigation using reliable antibody-based assays. Commercial DDX49 antibodies are typically developed using immunogenic peptides or recombinant protein fragments, with validation data crucial for experimental accuracy.
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