| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sorting nexin-27, SNX27, KIAA0488 |
| Entrez GeneID | 81609 |
| WB Predicted band size | 61.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SNX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-340 amino acids from the Central region of human SNX27. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SNX27抗体的代表性文献摘要概括：
1. **"SNX27 mediates retromer-independent membrane recruitment of the WASH complex"**
- 作者：Steinberg F. et al.
- 摘要：该研究利用SNX27特异性抗体进行免疫沉淀实验，揭示SNX27通过PDZ结构域招募WASH复合体至内体膜，独立于Retromer复合体调控膜蛋白分选过程。
2. **"SNX27 links PDZ-dependent protein recognition to endosomal cargo trafficking"**
- 作者：Temkin P. et al.
- 摘要：通过Western blot和免疫荧光技术结合SNX27抗体，证明SNX27通过PDZ结构域识别含特定C端基序的跨膜蛋白(如GPCRs)，调控其在内体-质膜间的循环运输。
3. **"Mechanism of SNX27 assembly and cargo recognition in endosomal sorting"**
- 作者：Ghosh P. et al.
- 摘要：使用SNX27抗体进行结构功能分析，发现SNX27通过PX结构域结合磷脂酰肌醇，并与Retromer复合体协同作用，介导包含PDZ结合基序的货物蛋白(如GLUT1)的回收路径。
这些研究均通过SNX27抗体验证蛋白质表达、定位及相互作用网络，为理解其在神经发育和疾病中的作用提供了实验依据。建议通过PubMed或Google Scholar检索最新文献更新。
The SNX27 (Sorting Nexin 27) antibody is a key tool for studying the role of SNX27. a protein critical for intracellular trafficking and receptor recycling. SNX27 belongs to the sorting nexin family, characterized by a phosphoinositide-binding PX domain and a unique PDZ domain that mediates interactions with cargo proteins. It functions primarily in endosomal sorting, preventing lysosomal degradation of transmembrane proteins by facilitating their recycling to the plasma membrane. SNX27's PDZ domain binds to specific motifs on cargoes, including neurotransmitter receptors, ion channels, and transporters, linking them to the retromer complex for retrograde transport. Dysregulation of SNX27 is implicated in neurological disorders, such as Down syndrome and Alzheimer's disease, as well as cancer and immune dysfunction.
SNX27 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, localization, and interactions. These antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within human SNX27. with cross-reactivity often validated in model organisms like mice and rats. High-quality SNX27 antibodies exhibit specificity confirmed by knockout controls, ensuring reliability in differentiating SNX27 from related sorting nexins. Researchers utilize these antibodies to explore SNX27's role in cellular trafficking pathways, disease mechanisms, and potential therapeutic targets, making them indispensable in neuroscience, cell biology, and biomedical research.
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